一种饵料微藻的脂肪酸甾醇分析及化学分类的探讨

徐继林; 严小军; 朱艺锋; 王晓申; 俞红

一种饵料微藻的脂肪酸甾醇分析及化学分类的探讨

宁波大学, 海洋生物工程重点实验室, 浙江, 宁波, 315211

基金项目: 国家新药基金资助项目(2003AA2Z3511);浙江省青年人才基金资助项目(RC02059);浙江省教育厅资助项目(20030455)

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出版历程
- 收稿日期: 2003-05-13
- 修回日期: 2004-02-06

Analysis of fatty acids and sterols in one marine feed microalga and its potential chemotaxonomic significance

Key Laboratory of Marine Biotechnology, Ningbo University, Ningbo 315211, China

摘要

摘要: 对一种重要的未知生物饵料微藻在充气和瓶中静态两种生态条件下进行培养,设计了一种分步衍生化脂肪酸与甾醇的GC/MS连续分析法,对微藻脂肪酸甾醇进行了定性和定量分析,结果表明,这种微藻主要含有丰富的二十碳五烯酸(EPA),C14:0,C16:0,C16:1以及一定量的二十二碳六烯酸(DHA),C22:5(n-6)和C18不饱和脂肪酸;共鉴定出11种甾醇组分,主要是24-甲基(乙基)胆甾醇,碳数分布从27到30,特别含有甲基巴夫甾醇和乙基巴夫甾醇;可以把这种微藻归到Prymnesiophyceae纲Pavlovales目Pavlovaceae科.通过实例分析证明,脂类分析结果可以作为海洋微藻化学分类学的一个重要依据.
- 脂肪酸 /
- 甾醇 /
- 海洋饵料微藻 /
- 化学分类学
Abstract: There is an unknown microalga(ZM2020) which has been used widely and proved to be nutritious as feed.In order to evaluate its nutrition value based on fatty acids and sterols,the alga has been cultured in two modes as standing and air bubbling conditions,simultaneo us analysis of fatty acids and sterols has been carried out on agas chromatography mass spectrometer by applying a new step-wise derivetization method.The results indicate that the microalg a is abundant in EPA,C14:0,C16:0,C16:1,as well as some conceivable amount of DH A,C22:5(n-6) and C18 unsaturated fatty acids.Eleven C27 to C30 sterols have been identified with major ones including 24-methyl(ethyl)-cholesterol.The existence of unusual sterols of methylpavlovol and ethyl pavlovol has been observed unam biguously.Based on the composition analysis of fatty acids and sterols,this alga can be classified into Prymnesiophy ceae,Pavlovales,Pavlo vaceae.It is also demonstrated here that lipid analysis can be a useful tool for microalgal chemotaxonomy.
- fatty acids /
- sterols /
- marine feed microalgae /
- chemotaxonomy

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参考文献(17)

BLIGH E G,DYER W J.A rapid methodlipid extraction and purification[J].Can J Biochem Physiol,1959,37(5):911-917.

詹益兴.实用气相色谱分析[M].湖南:湖南科学出版社,1990.169-181.

GHOSH P, PATTERSON G W, WIKFORS G H. Sterols in some marine Prymnesiophyceae[J]. J Phycol, 1998, 34(4):511-514.

VOLKMAN J K, FARMER C L, BARRETT S M. Unusual dihydroxysterols as chemotaxonomic markers for microalgae from the order Pavlovales(Haptophyceae)[J]. J Phycol,1997, 33(9):1 016-1 023.

曾志,曾陇梅.甾醇及其衍生物的质谱学规律[J].质谱学报,1994,15(2):11-16.1996,17(3):25-29.1996,17(4):13-17.

GLADU P K, PATTERSON G W, WIKFORS G H, et al. Free and combined sterols of Pavlova gyrans[J]. Lipids, 1991, 26(7):656-659.

LEWIS T E, NICHOLS P D, MCMEEKIN T A. Sterol and squalene content of a docosahexaenoic-acid-producing thraustochytrid:influence of culture age, temperature, and dissolved oxygen[J]. Marine Biotechnology, 2001,3(5):439-447.

LEBLOND J D, CHAPMAN P J. A survey of the sterol composition of the marine dinoflagellates Karenia brevis, Karenia mikimotoi, and Karlokinium micrum:distribution of sterols within other members of the class dinophyceae[J]. J Phycol, 2002, 38(5):670-682.

WIKFORS G H, GLADU P K, PATTERSON G W. In search of the ideal diet for oysters:recent progress with emphasis on sterols[J].J Shellfish Res, 1991,10(3):292-301.

HOLDEN M J, PATTERSON G W. Absence of sterol biosynthesis in oyster tissue culture[J]. Lipids, 1991,26(1):81-82.

WIKFORS G H, PATTERSON G W, GHOSH P, et al. Growth of post-set oysters, Crassostrea verginica, on high lipid strains of algal flagellates Tetraselmis spp[J]. Aquaculture. 1996,143(3):411-419.

徐继林,严小军.脂类分析在海洋微藻化学分类学上的研究进展[J].海洋通报,2004,23(2):65-72.

杨秀霞,于浩,曾晓起.影响微藻脂肪酸组成因素概述[J].海洋湖沼通讯,2001,32(1):76-81.

VOLKMAN J K, BARRETT S M, BLACKBURN S I, et al. Microalgae biomarkers:a review of recent research developments[J]. Qrg Geochem, 1998, 29(5-1):1 163-1 179.

VOLKMAN J K. Sterols in microorganisms[J]. Appl Microbiol Biotechnol, 2003, 60(4):495-506.

HALLEGRAEFF G M, NICHOLS P D, VOLKMAN J K, et al. Pigments,fatty acids,and sterols of the toxic dinoflagellate Gymnodinium catenatum[J]. Journal of Phycol, 1991, 27(5):591-599.

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