半胱胺盐酸盐和LHRH-A对黄鳍鲷生长激素分泌的影响

石和荣; 张为民; 刘晓春; 柯浩; 林浩然

半胱胺盐酸盐和LHRH-A对黄鳍鲷生长激素分泌的影响

1.
中山大学, 水生经济动物研究所, 广东省水生经济动物良种繁育重点实验室, 广东, 广州, 510275;广东省农科院, 兽医研究所, 广东, 广州, 510640
2.
中山大学, 水生经济动物研究所, 广东省水生经济动物良种繁育重点实验室, 广东, 广州, 510275
3.
广东省农科院, 兽医研究所, 广东, 广州, 510640

基金项目: 广东省科技厅农业攻关项目资助(C20710);国家自然科学基金农业倾斜项目资助(39970586);广东省科技计划项目资助(A3050261);教育部科学技术研究重点项目资助(02150)

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出版历程
- 收稿日期: 2004-03-03
- 修回日期: 2004-08-11

Effects of cysteamine hydrochloride and luteinizing hormone-releasing hormone analog on growth hormone secretion in Yellow-Fin Porgy

1.
Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou 510275, China;Veterinary Medicine Institute Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
2.
Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou 510275, China
3.
Veterinary Medicine Institute Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

摘要

摘要: 研究了生长抑素抑制剂半胱胺盐酸盐(CSH)和促黄体素释放激素类似物(LHRH-A)对黄鳍鲷生长激素(GH)分泌的影响.黄鳍鲷垂体GH含量在单独注射不同剂量CSH(25,50,100μg/gb.w)后2h均显著提高,注射后24h恢复正常.黄鳍鲷血清中GH水平在注射CSH(100μg/gb.w)后2h和CSH(25,50μg/gb.w)后6h均明显提高.单独注射LHRH-A(0.1μg/gb.w)后2h黄鳍鲷垂体GH含量和血清中GH水平均明显提高.注射CSH和LHRH-A后6h垂体GH含量明显高于单独注射CSH或LHRH-A,而血清GH水平在注射后12h明显高于单独注射CSH或LHRH-A.结果表明CSH和LHRH-A都能明显刺激黄鳍鲷垂体合成和释放GH,并且与CSH作用存在剂量依存关系,CSH和LHRH-A联合注射比单独注射作用更明显,作用的时间更持久.
- 半胱胺盐酸盐 /
- 促黄体素释放激素类似物 /
- 黄鳍鲷 /
- 生长激素
Abstract: Effects of cysteamine hydrochloride (a somatostatin-inhibiting agent,CSH) and luteinizing hormone-releasing hormone analog on growth hormone secretion in Sparus latus were investigated by i.p.injection.The pituitary GH contents of Sparus latus were significantly increased 2 hposti.p.injection of CSH with doses of 25,50 and 100 g/gb.w,and returned to control levels 24 h post injection.The serum GH levels of Sparus latus were significantly increased 2 h post injection of CSH with dose of 100 g/gb.w,and 6 h post injection of CSH with doses of 25 and 50 g/gb.w; LHRH- A(0.1 g/gb.w) significantly increased pituitary GH contents and serum GH levels 2 h post injection.Combining injection of LHRH-A and CSH significantly increased pituitary GH contents 6 h post injection as compared with CSH or LHRH-A alone,and the serum GH levels were significantly increased 12 h post injection as compared with CSH or LHRH-A alone.These results indicate that both CSH and LHRH-A could stimulate the synthesis and release of GH in Sparuslatus,and the effects of CSH were dose dependent.The combined effects of CSH and LHRH-A were significantly higher than those of CSH or LHRH-A alone.
- cysteamine hydrochloride(CSH) /
- LHRH-A /
- Sparus latus /
- growth hormone

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