RSM方法用于产红色素发酵培养基优化

武洪庆; 田黎; 张久明; 温占波

RSM方法用于产红色素发酵培养基优化

1.
国家海洋局, 海洋生物活性物质重点实验室, 山东, 青岛, 266061;河北工业大学, 天津, 300130
2.
国家海洋局, 海洋生物活性物质重点实验室, 山东, 青岛, 266061;青岛科技大学, 山东, 青岛, 266042
3.
国家海洋局, 海洋生物活性物质重点实验室, 山东, 青岛, 266061

基金项目: 国家“863”资助项目(2001AA620402);国家海洋局科技兴海资助项目(2000112);青岛市科技重点资助项目(JXM-0111)

计量
- 文章访问数: 761
- HTML全文浏览量: 14
- PDF下载量: 1720
- 被引次数: 0
出版历程
- 收稿日期: 2003-03-18
- 修回日期: 2003-06-09

Culture medium optimization for pigment production with RSM method

1.
Ley Lab oratory of Marine Biological Active Substance, First Institute of Oceanography, Qingdao 266061, China;Hebei Univerity of Technology, Tianjin 300130, China
2.
Ley Lab oratory of Marine Biological Active Substance, First Institute of Oceanography, Qingdao 266061, China;Qindao University of Science and Technology, qindao 266042, Chian
3.
Ley Lab oratory of Marine Biological Active Substance, First Institute of Oceanography, Qingdao 266061, China

摘要

摘要: 用RSM方法对黄杆菌属产红色素菌株S-9801(Flavobacterium sp.)的培养基进行了优化.首先用部分重复因子实验对培养基组分蛋白胨、酵母粉、葡萄糖及NaCl浓度对菌株产红色素的影响进行评价,找出主要影响因子为葡萄糖和NaCl浓度.两者均为正影响,其他组分对色素含量的影响不显著.第二步用最陡爬坡路径逼近最大响应区域.最后用中心组合设计及响应面分析确定主要影响因子的最佳浓度.菌株在优化培养基中培养较初始培养基色素含量提高320%.
- RSM方法 /
- 培养基优化 /
- 色素
Abstract: Response surface methodology was used to optimize a medium for pigment production of marine bacterium S-9801 strain.In the first optimization step the influence of yeast extract,peptone,glucose and sodium chloride on pigment production was evaluated using a fractional factorial design.Glucose and sodium chloride influenced pigment production positively while other components had no significant effect.The path of steepest ascent was used to approach the optimal region of the medium composition.In the third step the optional concent ration of glucose and sodium chloride was determined by a central composite design and response surface analysis.The optimized medium allowed pigment production(OD_535~650) to be in creased from 463 to 559,320% higher than the original medium.
- response surface methodology /
- red pigment /
- optimization

HTML全文

参考文献(9)

TIAN Li,HE Pei-qing,WU Hong-qing,et al.Biological characteristics of marine bacterium S-9801 strain and its culture conditions of pigment production[J].Acta Oceanologica Sinica,2002,21(4):541-545.

TIAN Li,HE Pei-qing,LIU Chen-lin,et al.Extraction and physicochemical characteristics of a red pigment produced by marine bacterium strain S-9801[J].Acta Oceanologica Sinica,2002,21(3):445-449.

SILVERMAN M P,MUNOZ E F.Effect of iron and salt on prodigiosin synthesis in Serratia marcescens[J].Journal of Bacteriology.1973,114(3):999-1 006.

陆玲,孙延涛,唐勇,等.一种放线菌发酵产天然蓝色素的研究[J].微生物学通报,2001,28(4):50-54.

李孱,白景华,蔡昭铃,等.细菌素发酵培养基优化及动力学初步分析[J].生物工程学报,1998,17(2):187-192.

欧红宇,贾士儒.SAS软件在微生物培养条件优化中的应用[J].天津轻工业学院学报,2001,(3):14-17.

褚以文.微生物培养基优化方法及OPTI优化软件[J].国外医药抗生索分册,1999,20(2):58-60.

洪楠,侯军.SASfor windows统计分析系统教程[M].北京:电子工业出版社,2001.

杜荣骞.生物统计学[M].北京:高等教育出版社,1999.

施引文献

资源附件(0)

访问统计