AFLP技术在笛鲷的仔鱼鉴定及其分类学上的研究

张俊彬; 黄良民; 陈真然

AFLP技术在笛鲷的仔鱼鉴定及其分类学上的研究

1.
中国科学院, 南海海洋研究所, 广东, 广州, 510301;中国科学院, 海洋研究所, 山东, 青岛, 266071
2.
中国科学院, 南海海洋研究所, 广东, 广州, 510301

基金项目: 国家自然基金资助项目(40306022);广东省自然科学基金(04001300)

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出版历程
- 收稿日期: 2003-10-06
- 修回日期: 2004-07-26

Studies on the larval identification and taxonomy of Lutjanus by AFLP technology

1.
South China Sea Institute of Oceanography, Chinese Academy of Sciences, Guangzhou 510301, China;Institute of Oceanography, Chinese Academy of Sciences, Qingdao 266071, China
2.
South China Sea Institute of Oceanography, Chinese Academy of Sciences, Guangzhou 510301, China

摘要

摘要: 以南沙群岛采获的勒氏笛鲷(Lutjanus russelli)、黄笛鲷(Lutjanus lutjanus)、红笛鲷(Lut janus sanguineus)、紫红笛鲷(Lutjanus argentimaculatus)、金焰笛鲷(Lutjanus fulviflamma)、线纹笛鲷(Lutjanus lineolatus)、画眉笛鲷(Lutjanus vitta)、马拉巴笛鲷(Lutjanus malabarius)、驼背笛鲷(Lutjanus gibbus)、约氏笛鲷(Lutjanus johni)、金带笛鲷(Lutjanus vaigiensis)11种笛鲷属的后期仔鱼为材料, 进行了基因组DNA的AFLP(扩增片段长度多态性)研究,通过笛鲷仔鱼和成鱼的AFLP电泳图谱的比较分析,将这11种笛鲷属的仔鱼成功分开.研究表明,最适合的AFLP引物组合是E+AGC/M+CAA,由这11种笛鲷共扩增出132 AFLP位点,每种笛鲷的AFLP条带数为43~69,在这11种笛鲷中,共享的AFLP条带数仅为7,同一种笛鲷仔鱼和成鱼AFLP遗传相似度在90%以上.根据这11种笛鲷的Nei遗传距离对Neighboring系统进行进化分析,并且与传统的笛鲷形态分类进行了比较.分子生物学可以阐释笛鲷属鱼类的系统进化和遗传亲缘关系,对传统形态分类学加以完善和补充.
- AFLP /
- 仔鱼鉴定 /
- 分类学 /
- 笛鲷属
Abstract: The Nansha Coral Reefs(also called Spratly Archipelago)water area,which is located in the South China Sea,is the main habitat and spawn area for the species of Lutjanus in China.The larvae of many closely related species,such as those of the genus Lutjanus,are different to be distinguished mor-phologically.APCR-based fingerprinting technology called amplified fragment length polymorphism (AFLP)was used in the characterization and identification of 11 species of the genus Lutjanus sampled from the Nansha Coral Reefs.The optimal AFLP pattern was obtained with primer combination of E+AGC/M+CAA selective nucleotides.Totally,132 AFLP loci were found from these species and the number of AFLP bands for each species varied from 43 to 69.More than 90% of AFLP bands were species-specific and only 7 markers were fixed to all species examined.All larvae were successfully identified to 11 Lutjanus species by comparing AFLP finger prints of larvae with that of adults.Moreover,the phylogenetic analysis(Neighboring)of species relationship based on the AFLP data was compared with the current morphological taxonomic data.It suggests that AFLP analysis is a reliable method for the larval identification and taxonomy of the genus Lutjanus.
- AFLP /
- larval identification /
- taxonomy /
- genus Lutjanus

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参考文献(21)

NEWMAN S J, CAPPO M, WILLIAMS D M. Age, growth and mortality of the stripey, Lutjanus carponotatus (Richardson) and the brown-stripe snapper, L. vitta (Quoy and Gaimard) from the central Great Barrier Reef[J]. Fish Res Australia, 2000, 48: 263-275.

MUSIY Y I, SERGIYENKO V A. Finerlings of the genus Lutjanus (Lutjanidae, Perciformes) from the Gulf of Aden[J]. J Ichthyol,1977, 17: 151-154.

张仁斋,陆淑芳,赵传铟,等.中国近海鱼卵和仔鱼[M].上海:上海科学技术出版社,1983.

OKIYAMA M. An Atlas ofthe Early Stage Fishesin Japan[M].Tokyo:East Sea University Press,1987.

刘振辉,孔杰,刘萍,等.RAPD技术在中国对虾群体鉴别上的应用[J].海洋学报,2003,23(4):67-71.

黎中宝,林鹏.我国不同纬度白骨壤种群遗传多样性和遗传分化的研究[J].海洋学报,2002,24(1):142-147.

周涵韬,林鹏.海桑属红树植物遗传多样性和引种关系研究[J].海洋学报,2002,24(5):98-106.

ALLEN G R. Genus Lutjanus. Margaret M Smith, Phillip C Heemstra. Smiths'Sea Fishes[M]. New York:Spriger-Verlag, 1986.

成庆泰,郑葆珊.中国鱼类系统检索[M].北京:科学出版社,1987.

NEI MASATOSHI, LI, WEN HSIUNG. Mathematical model for studying genetic variation in terms of restriction endonucleases[J].Proc Natl Acad Sci USA,1979, 76: 5 259-5 273.

HULATA G. Israel. Pullin R S.V. Tilapia Genetic Resources for Aquaculture[M]. ICLARM Conf Pro 16, Manilia, Philippines. Penang:World Fish Fress, 1998. 48-51.

AGRESTI J J, SEKI S, CNAANI A, et al. Breeding new strains of tilapia: development of an artificial center of origin and linkage map based on AFLP and microsatellite loci[J]. Aquaculture, 2000, 185: 43-56.

ASAHIDA T, YAMASHITA Y,KOBAYASHI T. Identification of consumed stone flouder, Kareius bicoloratus (Basilewsky), from the stomach contents of sand shrimp, Crangon a f finis (De Haan) using mitochondrial DNA analysis[J]. J Exp Mar Biol Ecol, 1997, 217:153-163.

ROCHA-OLIVARES A. Multiplex haplotype-specific PCR: a new approach for species identification of the early life stages of rockfishes of the species-rich genus Sebastes Cuvier[Ⅰ]. Ⅰ Exp Mar Biol Ecol, 1998,231:279-290.

SEBASTIO PZP, NERI TM. Identification of anchovy (Engraulis encrasicolus L.) and gilt sardine (Sardinella aurita) by polymerase chain reaction, sequence of their mitochondrial cytochrome b gene,and restriction analysis of polymerase chain reaction products in semipreserves[J]. J Agric Food Chem, 2001,49 (3): 1 194-1 199.

VOS P, HOGERS M, REIJIANS M, et al. AFLP: a new technique for DNA fingerprinting[J]. Nucleic Acids Res, 1995,23:4 407-4 414.

THOME J, GONZALEZ D O, BEEBE S, et al. AFLP analysis of gene pools of a wild bean core collection[J]. Crop Sci, 1996, 36:1 375-1 384.

JOHNSON M S, HEBBERT D R, MORAN M J. Genetic analysis of population of north-western Australian fish species[j]. Aust JMar Freshwat Res, 1993, 44: 673-685.

GOLD J R, RICHARDSON L R. Mitochondrial DNA variation among ‘red, fishes from the Gulf of Mexico[J]. Fish Res, 1994, 20:137-150.

CAMPER J D, BARBER R C, RICHARDSO)N L R, et al. Mitochondrial DNA variation among red snapper (Lutjanus campechanus)from the Gulf of Mexico[J]. Mol Mar Biol Biotechnol,1993, 3: 154-161.

LIEVENS S, GOORMACHTIG S, HOLSTERS M. A critical evaluation of differential display as a tool to identify genes involved in legume nodulation: looking back and looking forward[J]. Nucleic Acids Res, 2001,29(17): 3 459-3 468.

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