4种养殖扇贝的群体遗传多样性及特异性AFLP标记研究

陈省平; 包振民; 潘洁; 胡景杰

4种养殖扇贝的群体遗传多样性及特异性AFLP标记研究

1.
中国海洋大学, 生命科学与技术学部, 山东, 青岛, 266003;中山大学, 广东, 广州, 510275
2.
中国海洋大学, 生命科学与技术学部, 山东, 青岛, 266003

基金项目: 国家“十五”“863”计划资助项目(2003AA603022,2002AA62110);山东省优秀中青年科学家科研奖励基金资助项目(01BS10)

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出版历程
- 收稿日期: 2004-04-20
- 修回日期: 2004-09-20

Analyses of the population genetic diversity and species specific markers in four scallop species, Patinopecten yessoensis, Argopecten irradians, Chlamys nobilis and C. farreri

1.
Ocean University of China, Qingdao 266003, China;Sun Yat-sen University, Guangzhou 510275, China
2.
Ocean University of China, Qingdao 266003, China

摘要

摘要: 应用AFLP技术对我国4种主要养殖扇贝进行了遗传分析.虾夷扇贝、海湾扇贝、华贵栉孔扇贝、栉孔扇贝4种养殖扇贝群体中,种内遗传相似度依次分别为0.841 5,0.786 3,0.719 0和0.673 1,香侬氏指数分别为43.52,58.87,80.16和92.83,栉孔扇贝和华贵栉孔扇贝的遗传多样性水平明显高于海湾扇贝和虾夷扇贝;AFLP标记共享位点分布表明,在4种扇贝中栉孔扇贝与华贵栉孔扇贝之间的共享位点最多,同时4种扇贝的种间遗传相似度也以栉孔扇贝和华贵栉孔扇贝之间为最高(0.769 5),海湾扇贝和虾夷扇贝之间为最低(0.662 6),揭示所研究的几种扇贝中栉孔扇贝和华贵栉孔扇贝的遗传关系最近(遗传距离为0.262 1);找到了21个种内特异性AFLP标记和种间共享标记,可用于种质鉴定和分子辅助分类.
- 扇贝 /
- AFLP /
- 分子标记 /
- 遗传多样性
Abstract: The AFLP technology was used to analyse the genetic diversity of four scallops,Patinopecten yessoensis,Argopecten irradians,Chlamys nobilis and C. farreri.The genetic similarity index of the four species is 0.841 5,0.786 3,0.719 0 and 0.673 1,while Shannon index is 43.52,58.87,80.16 and 92.83, respectively.The results show that the two native species,C. farreri and C. nobilis,have higher level of the genetic diversity than the two introduced species,A. irradians and P. yessoensis.The results also show that C. nobilis and C. farreri shared the most commonloci.The genetic distance indicates that C. nobilis and C. farreri are closely related.Moreover,out of 510 AFLP markers,21 specific bands are found to distinguish the four species scallop and these markers may be applied for germplasm charact erization and molecular assistant classification in scallops.
- scallop /
- AFLP /
- markers /
- genetic diversity

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