坛紫菜别藻蓝蛋白α, β亚基基因的克隆和序列分析

左正宏; 邓元告; 陈奕欣; 赵扬; 郑微云

坛紫菜别藻蓝蛋白α, β亚基基因的克隆和序列分析

1.
厦门大学, 生命科学学院, 细胞生物学与肿瘤细胞工程教育部重点实验室, 福建, 厦门, 361005;厦门大学, 环境科学研究中心, 福建, 厦门, 361005
2.
厦门大学, 生命科学学院, 细胞生物学与肿瘤细胞工程教育部重点实验室, 福建, 厦门, 361005
3.
厦门大学, 环境科学研究中心, 福建, 厦门, 361005

基金项目: 福建省重大农业科技资助项目(2001Z017);国家海洋“863”资助项目(2002AA603023)

计量
- 文章访问数: 829
- HTML全文浏览量: 8
- PDF下载量: 1108
- 被引次数: 0
出版历程
- 收稿日期: 2003-08-26
- 修回日期: 2004-03-08

Cloning and sequence analysis of allophycocyanin genes from Porphyra haitanensis

1.
Key Laboratory of Ministry of Education on Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China;Environmental Science Research Center, Xiamen University, Xiamen 361005, China
2.
Key Laboratory of Ministry of Education on Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China
3.
Environmental Science Research Center, Xiamen University, Xiamen 361005, China

摘要

摘要: 以野生坛紫菜色素体DNA为模板, 通过PCR扩增获得编码坛紫菜别藻蓝蛋白α亚基和β亚基的序列及两者之间的间隔序列,该序列全长为1 055 bp,其中编码α和β亚基的序列均为486 bp,间隔序列为83 bp.该序列与GenBank收录的其他4种红藻相关序列的同源性在75.6%~87.4%,与2种蓝藻的同源性分别为66.9%,68.5%,其中编码区同源性更高.
- 坛紫菜 /
- 别藻蓝蛋白 /
- 基因克隆 /
- 序列分析
Abstract: On the basis of the sequences of allophycocyanins from several species in genbank,a pair of degenerate primers were designed and synthesized.Aspecific fragment about 1 055 bp in size was obtained after PCR amplification using the cpDNA of Porphyra haitanensis as template.Then the purified fragment of DNA was cloned into vector pMD18-T,and sequenced after the identification of the recombinants using PCR and endonuclease digest.The results indicate that A and B subunit genes of allophy cocyanin are obtained from Porphyra haitanensis.The fragment size is 1 055 bp,including A and B subunits coding regions 486 bp each and the spacer between them is 83 bp.The genes were submitted to genbank and their genbank accession number was AY372218.
- Porphyra haitanensis /
- allophycocyanin /
- gene cloning /
- sequence analyzing

HTML全文

参考文献(11)

GLAZER A N, LUNDELL J, YAMANAKA G, et al. The structure of a simple phycobilisome[J]. Ann Microbiol, 1983,134:159-180.

CANTT E. Structure and function of phycobilisomes: light harvesting pigment complexes in red and blue green algae[J]. Iht Rev Cyt,1980, 66:45-80.

SHINOHARA K, OKURA Y, KOYANO T, et al. Algal phycocyanins promote growth of human cells in culture[J]. In Vitro Cell DevBiol, 1988, 24(10):1 057-1 060.

SHIH S R, TSAI K N, LI YI SHUANG, et al. Inhibition of enterovirus 71-induced apoptosis by allophycocyanin isolated from a bluegreen alga Spirulina platensis[J]. J Med Virol, 2003, 70(1): 119-25.

俞丽君,李永明,陈艳丽,等.钝顶螺旋藻藻胆蛋白的纯化及其清除自由基的作用[J].台湾海峡,1999,18(2):172-176.

QIN Song. The Marine Biology of South China Sea[M]. Hong Kong: Hong Kong University Press, 1997. 3-11.

唐书明,黄芳,窦昌贵,等.基因重组别藻蓝蛋白对小鼠S180肉瘤的抑制作用[J].药物生物技术,1999,6(3):168-170.

赵方庆,唐志红,林凡,等.表达rAPC大肠杆菌的高密度发酵及纯化产物的抑瘤活性[J].高技术通讯,2003,13(2):29-33.

DRAGANA S,MARCOAURELIO A, CF SAR P, et al. Chloroplast isolation from Laminaria digitata (Phaeophyceae): a reproducible methodology yielding photosynthetically active chloroplasts[J]. Planta, 1994,195:138-141.

萨姆布鲁克,拉塞尔.分子克隆操作指南(第三版)[M].黄培堂,等译.北京:科学出版社,2002.213-218.

郭宝太,毕玉平,单雷,等.条斑紫菜高纯度总DNA及其质粒状DNA的提取[J].海洋学报,2000,22(3):87-91.

施引文献

资源附件(0)

访问统计