坛紫菜别藻蓝蛋白α, β亚基基因的克隆和序列分析

左正宏; 邓元告; 陈奕欣; 赵扬; 郑微云

坛紫菜别藻蓝蛋白α, β亚基基因的克隆和序列分析

1.
厦门大学, 生命科学学院, 细胞生物学与肿瘤细胞工程教育部重点实验室, 福建, 厦门, 361005;厦门大学, 环境科学研究中心, 福建, 厦门, 361005
2.
厦门大学, 生命科学学院, 细胞生物学与肿瘤细胞工程教育部重点实验室, 福建, 厦门, 361005
3.
厦门大学, 环境科学研究中心, 福建, 厦门, 361005

基金项目: 福建省重大农业科技资助项目(2001Z017);国家海洋“863”资助项目(2002AA603023)

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出版历程
- 收稿日期: 2003-08-26
- 修回日期: 2004-03-08

Cloning and sequence analysis of allophycocyanin genes from Porphyra haitanensis

1.
Key Laboratory of Ministry of Education on Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China;Environmental Science Research Center, Xiamen University, Xiamen 361005, China
2.
Key Laboratory of Ministry of Education on Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China
3.
Environmental Science Research Center, Xiamen University, Xiamen 361005, China

摘要

摘要: 以野生坛紫菜色素体DNA为模板, 通过PCR扩增获得编码坛紫菜别藻蓝蛋白α亚基和β亚基的序列及两者之间的间隔序列,该序列全长为1 055 bp,其中编码α和β亚基的序列均为486 bp,间隔序列为83 bp.该序列与GenBank收录的其他4种红藻相关序列的同源性在75.6%~87.4%,与2种蓝藻的同源性分别为66.9%,68.5%,其中编码区同源性更高.
- 坛紫菜 /
- 别藻蓝蛋白 /
- 基因克隆 /
- 序列分析
Abstract: On the basis of the sequences of allophycocyanins from several species in genbank,a pair of degenerate primers were designed and synthesized.Aspecific fragment about 1 055 bp in size was obtained after PCR amplification using the cpDNA of Porphyra haitanensis as template.Then the purified fragment of DNA was cloned into vector pMD18-T,and sequenced after the identification of the recombinants using PCR and endonuclease digest.The results indicate that A and B subunit genes of allophy cocyanin are obtained from Porphyra haitanensis.The fragment size is 1 055 bp,including A and B subunits coding regions 486 bp each and the spacer between them is 83 bp.The genes were submitted to genbank and their genbank accession number was AY372218.
- Porphyra haitanensis /
- allophycocyanin /
- gene cloning /
- sequence analyzing

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参考文献(11)

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