冠瘤海鞘幼体附着和变态的离子控制

黄英; 柯才焕; 冯丹青; 周时强; 李复雪

冠瘤海鞘幼体附着和变态的离子控制

1.
厦门大学, 海洋系, 厦门大学, 福建, 厦门, 361005;厦门大学, 亚热带海洋研究所, 福建, 厦门, 361005;M厦门大学, 海洋环境科学教育部重点实验室, 福建, 厦门, 361005
2.
厦门大学, 海洋系, 厦门大学, 福建, 厦门, 361005;厦门大学, 亚热带海洋研究所, 福建, 厦门, 361005;M

基金项目: 国家自然科学基金资助项目(49976034)

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出版历程
- 收稿日期: 2002-12-25
- 修回日期: 2003-04-22

Ionic control of settlement and metamorphosis in the tadpole larvae of ascidian, Styela canopus Savigny

1.
Department of Oceanography, Xianmen Unitersity, Xiamen 361005, China;Institute of Subtropic Oceanography, Xiamen University, Xiamen 361005, China;Key Laboratory of Marine Environmental Science of the Ministry of Education, Xiamen University, Xiamen 361005, China
2.
Department of Oceanography, Xianmen Unitersity, Xiamen 361005, China;Institute of Subtropic Oceanography, Xiamen University, Xiamen 361005, China

摘要

摘要: 研究了人工海水中三种金属离子K⁺,Mg²⁺和Ca²⁺分别在不同浓度下对冠瘤海鞘(Styelaca nopusSavigny)幼体附着和变态的影响,同时采用K⁺通道阻滞剂TEA检验其可兴奋细胞膜上的K⁺通道特性,对冠瘤海鞘幼体附着和变态的离子控制机制进行初步探讨,结果表明:K⁺的浓度为20~40mmol/dm³时诱导幼体附着和变态的效果最佳,低于45 mmol/dm³和高于80mmol/dm³的K⁺浓度都表现出抑制效果;Mg²⁺浓度在0~32 mmol/dm³时显著促进幼体附着和变态,达88mmol/dm³时起抑制作用;在人工海水中无论添加或减少Ca²⁺,均有效抑制幼体变态.TEA不能阻抑增加K⁺的诱导作用,这表明冠瘤海鞘幼体可兴奋细胞膜上的K⁺通道对外部TEA不敏感.另外,人工海水中缺乏K⁺或Ca²⁺或K⁺浓度达80mmol/dm³都会对冠瘤海鞘幼体产生毒害作用.
- 冠瘤海鞘 /
- 幼体 /
- 附着 /
- 变态 /
- 离子控制 /
- 海鞘
Abstract: The effects of changes in concentrations of potassium,magnesium and calcium on settlement and metamorphosis in the Styela canopus Savigny larvae in artifical seaw ater and the characteristic of the potassium channels in externally accessible,excitable cell mem brane were studied.K⁺ effectively induced larval settlement and metamorphosis during the concentrations of 20~40 mmol/dm³,but showed inhibitive effect in the concentrations being less than or egual to 4.5 mmol/dm³ or being greater than or egual to 80 mmol/dm³.The inductive effect of 0~32 mmol/dm³ Mg²⁺ was remarkable,but 88 mmol/dm³ Mg²⁺ inhibited larval settlement and metamorphosis.The settlement and metamorphosis of the larvae would be inhibited no matter whether increased or decreased Ca²⁺ in artificial seawater.The failure of inhibiting the inductive effect of K⁺ by a K⁺ -channel blocker TEA (tetraethylamm onium chloride) indicated that the potassium channe ls in externally accessible,excitable cellmem brane of Styela canopus Savigny larvae are insensitive to external TEA.Like 80 mmol/dm³ K⁺,K⁺ -free or Mg²⁺-free sea waterw ould produce evidence of toxicity.The mechanisms of ionic control of settlement andmetamorphosis in the Styela canopus Savigny larvae were discussed.
- Styela canopus Savigny /
- larvae /
- settlement /
- metamorphosis /
- ionic control /
- ascidian

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参考文献(35)

CHIA F S.Settlement and metamorphosis of marine invertebrate larvae[M].London:Elsevier,1978.283-286.

MORSE D E.Recent progress in larval settlement and metamorphosis:closing the gaps between molecular biology and ecology[J].Bull Mar Sci,1990,46:465-483.

柯才焕.海洋贝类幼体附着和变态的化学诱导研究进展[J].海洋通报,1993,12(3):107-116.

柯才焕,李少菁,李复雪,等.翡翠贻贝幼体附着和变态的离子控制[J].海洋与湖沼,1998,29(2):128-135.

BALOUN A J,MORSE D E.Ionic control of settlement and metamorphosis in larval Haliotis rufescens (Gastropoda) [J].Biol Bull,1984,167:124-138.

SV ANE I,YOUNG C M.The ecology and behaviour of ascidian Larvae[J].Oceanogr Mar Biol,1989,27:45-90.

DEGNAN B M,SOUTHER D,DEGNAN S M,et al.Induction of metamorphosis with potassium ions requires development of competence and an anterior signaling centre in the ascidian Herdmania momus[J].Dev Genes Evol,1997,206:370-376.

GRAVE C,NICOLL P A.Studies of larval life and metamorphosis in Ascidia nigra and species of Polyandrocarpa [J].Carnegie Inst Wash Pub,1939,517:1-46.

GLASER O,ANSLOW G A.Copper and ascidian metamorphosis [J].J Exp Zool,1949,111:117-140.

WHITTAKER J R.Copper as a factor in the onset of ascidian metamorphosis[J].Nature,1964,202:1 024-1 025.

BRADWAY W.The experimental alteration of the rate of metamorphosis in the tunicate,Clavelina huntsmani (Van Name) [J].J Exp Zool,1936,72:213-225.

BERTHOLF L M.Accelerating metamorphosis in the tunicate,Styela partita(abstract) [J].Biol Bull,1945,89:184-185.

BERKING S,HERRMANN K.Dicapryloylglycerol and ammol/Lonium ions induce metamorphosis of ascidian larvae [J].Roux' s Arch Dev Biol,1990,198:430-432.

赖利J P,斯基罗G.刘光译.化学海洋学[M].北京:海洋出版社,1982.47-344.

YOOL A J,GRAU S M,HADFIELD M G,et al.Excess potassium induces larval metamorphosis in four marine invertebrate species[J].Biol Bull,1986,170:255-266.

PECHENIK J A,HEYMAN W D.Using KCl to determine size at competence for larvae of the marine gastropod Crepidula fornicate(L.) [J].J Exp Mar Biol Ecol,1987,112:27-38.

HUBBARD E J A.Larval growth and the induction of metamorphosis of a tropical sponge-eating nudibrach[J].J Moll Stud,1988,54:249-269.

柯才焕,李少菁,李复雪,等.两种东风螺幼虫附着和变态的化学诱导研究[J].海洋学报,1996,18(4):90-95.

柯才焕.儿茶酚胺对翡翠贻贝幼体附着和变态的诱导[J].厦门大学学报,1995,34(6):975-981.

刘保忠,张福绥,何朝义.几种神经活性物质对海湾扇贝幼虫变态诱导作用的研究[J].海洋学报,1998,20(5):55-60.

柯才焕,孙泽伟,周时强,等.华贵栉孔扇贝幼体附着和变态的化学诱导[J].海洋科学,2000,24(12):5-8.

NELL J A,HOLLIDAY J E.Effect of potassium and copper on the settling rate of Sydney rock eyster(Saccastrea commol Lercialis) larvae [J].Aquaculture,1986,58:263-267.

PAWLIK J R.Natural and artificial induction of metamorphosis of Phragmatopoma lapidosa californica (Polychaeta:Sabellariidae),with a critical look at the effects of bioactive compounds on marine invertebrate larvae[J].Bull Mar Sci,1990,46(2):512-536.

STRICKE S A.Settlement and metamorphosis of the marine bryozoan Membranipora membranacea[J].Bull Mar Sci,1989,95:139-152.

MORSE D E.Neurotransmitter-mimetic inducers of larval settlement and metamorphosis[J].Bull Mar Sci,1985,37:697-706.

RITTSCHOF D,MAKI J,MITCHELL R,et al.Ion and neuropharmacological studies of barnacle settlement[J].Netherlands J Sea Research,1986,20:269-275.

EYSTER L S,PECHENIK J A.Attachment ofMytilus edulis L.larvae on algal and byssal filaments is enhanced by water agitation[J].J Exp Mar Biol Ecol,1987,114:99-110.

KOSTYUK P G,DOROSHENKO P A,Tsyndrenko.calcium-dependent potassium conductance studied on internally dialyzed nerve cells [J].Neuroscience,1980,5:187-192.

EDGINGTON D R,STUART A E.Properties of tetraethylammol/Lonimn ion-resistant K⁺ channels in the photoreceptor membrane of the giant barnacle[J].J Gen Physiol,1981,77:629-646.

ARMSTRONG C M,BINSTOCK L.Anomalous rectification in the squid giant axon injected with tetraethylammol/Lonium chloride[J].J Gen Physiol,1965,48:859-872.

HERMANN A,GORMAN A L F.External and internal effects of tetraethylammol/Lonium on voltage-dependent and Ca-dependent K⁺ currents components in molluscan pacemaker neurons[J].Neurosci Lett,1979,12:87-92.

HERMANN A,GORMAN A L F.Effects of tetraethylammol/Lonium on potassium currents in a molluscan neuron[J].J Gen Physiol,1981,78:87-110.

LYNCH W F.Extrinsic factors influencing metamorphosis in bryozoan and ascidian larvae[J].Am Zool,1961,1:59-66.

黄英,柯才焕,周时强.国外对藤壶幼体附着的研究进展[J].海洋科学,2001,25(3):30-32.

YAMAMOTO H,TACHIBANA A,MATSUMURA K,et al.Protein kinase C (PKC) singal transduction system involved in larval metamorphosis of the barnacle,Balanus amphitrite[J].Zool Sci(Tokyo),1995,12:391-396.

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