皱纹盘鲍杂交F<sub>1</sub>代与亲本的RAPD标记及分离方式分析

肖洁; 刘晓; 张国范; 郭希明

皱纹盘鲍杂交F₁代与亲本的RAPD标记及分离方式分析

1.
中国科学院, 海洋研究所, 山东, 青岛, 266071
2.
中国科学院, 海洋研究所, 山东, 青岛, 266071;Haskin贝类研究实验室, 新泽西州立大学, 新泽西, 08349

基金项目: 国家"863"计划课题资助项目(2002AA629030;2001AA621070);国家自然科学基金资助项目(30371117;39825121).

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出版历程
- 收稿日期: 2004-03-29
- 修回日期: 2004-07-13

Studies on segregation of RAPD markers in a F₁ hybrid family and parents of Haliotis discus hannai Ino

1.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Haskin Shellfish Research Laboratory, Rutgers University, Port Norris, NJ 08349, USA

摘要

摘要: 应用RAPD标记技术对皱纹盘鲍杂交家系JC的双亲及3个F₁个体进行了分析,对JC家系在皱纹盘鲍遗传连锁图谱构建中的应用前景进行了初步评估,并分析了RAPD分子标记在JC家系F₁代中的分离方式及该家系的1对亲本及其子代个体的遗传结构.7条随机引物共扩增出41个分子标记,其中24个为多态性,占总数的58.5%;有14个分离标记可用于遗传连锁作图,有7个符合孟德尔独立分配定律,占总数的17.1%.2个标记与子代个体大小有一定的相关性,但其连锁关系仍有待于进一步研究.父母本之间的遗传距离为0.384 4,父本与子代间的距离为0.137 2,母本与子代间的距离为0.127 3;父本提供的分离标记在子代中的显性频率为0.47,母本为0.55.双亲之间的遗传距离较远,杂交产生的JC-F₁群体具有较高的杂合度(0.201 6),该结果初步表明皱纹盘鲍杂交家系JC的子1代即可作为作图群体应用于皱纹盘鲍的连锁图谱构建.
- 皱纹盘鲍 /
- RAPD /
- 遗传结构 /
- 分子标记 /
- 连锁图谱
Abstract: The genetic structure and segregation of molecular markers in a F₁ hybrid family of Pacific abalone(Haliotis discus hannai Ino)were studied using random amplified polymorphic DNA(RAPD)technology.This family's usefulness in linkage mapping of abalones was assessed.Seven primers yielded a total of 4.markers,of which 24 (58.5%)were polymorphic in this family,14 were informative and could be used for linkage mapping,and 7 (17.1%)markers were segregated in the expected Mendelian ratio which is very low compared with other species. Two markers show some association with JC-F₁'s phenotypes,and this relationship needs further study.The genetic distances between two parents,father and progeny,mother and progeny are 0.384 4,0.137 2,0.127 3 respectively.The frequency in JC-F₁ population of RAPD markers from father and mother are 0.824 and 0.841, which are almost the same.High genetic distance between parents and high heterozygosity(0.2016)in JC-F₁ population is also confirmd the feasibility of linkage mapping using these parents and progenies.
- Haliotis discus hannai /
- RAPD /
- genetic structure /
- molecular markers /
- linkage mapping

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皱纹盘鲍杂交F₁代与亲本的RAPD标记及分离方式分析

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Studies on segregation of RAPD markers in a F₁ hybrid family and parents of Haliotis discus hannai Ino

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皱纹盘鲍杂交F1代与亲本的RAPD标记及分离方式分析

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Studies on segregation of RAPD markers in a F1 hybrid family and parents of Haliotis discus hannai Ino

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出版历程

目录

皱纹盘鲍杂交F₁代与亲本的RAPD标记及分离方式分析

Studies on segregation of RAPD markers in a F₁ hybrid family and parents of Haliotis discus hannai Ino