细基江蓠及其繁枝变种的RAPD和ITS分析

李文红; 胡自民; 覃志彪; 郭亚芬; 王如才; 段德麟

细基江蓠及其繁枝变种的RAPD和ITS分析

1.
中国海洋大学, 水产学院, 山东, 青岛, 266003;广西大学, 水产系, 广西, 南宁, 530005;中国科学院, 海洋研究所, 山东, 青岛, 266071
2.
中国海洋大学, 水产学院, 山东, 青岛, 266003
3.
广西大学, 水产系, 广西, 南宁, 530005
4.
中国科学院, 海洋研究所, 山东, 青岛, 266071

基金项目: 国家"863"高技术发展计划资助项目(2001AA621090);广西科学基金资助项目(0236011);青岛市科技局资助项目(03-2-JSH-02).

计量
- 文章访问数: 1038
- HTML全文浏览量: 20
- PDF下载量: 1036
- 被引次数: 0
出版历程
- 收稿日期: 2004-04-08
- 修回日期: 2004-06-30

RAPD detection and ITS sequences analysis of Gracilaria tenuistipitata and G.tenuistipitata var. liui

1.
Fisheries College Ocean University of China, Qingdao 266003, China;University of Guangxi, Nanning 530005, China;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2.
Fisheries College Ocean University of China, Qingdao 266003, China
3.
University of Guangxi, Nanning 530005, China
4.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

摘要

摘要: 对细基江蓠及其繁枝变种各12个个体进行随机扩增多态性(RAPD)DNA分析,对比多态位点比例、平均杂合度以及遗传距离,并构建UPGMA聚类图;通过PCR扩增出核糖体RNA基因(rDNA)转录间隔区(ITS),纯化后直接测序,利用生物信息学方法进行序列分析和核苷酸变异研究.比较分析结果表明,细基江蓠的杂合度和多态位点比例均高于细基江蓠繁枝变种,其遗传多样性相对较丰富;实验中8条随机引物扩增出特异的RAPD带,可用做细基江蓠及其繁枝变种的分子鉴定标记;ITS序列在这两种海藻中变异极小;RAPD和ITS聚类分析研究结果表明,细基江蓠及其繁枝变种均聚集为一枝,与同属不同种的龙须菜和真江蓠分开,提示细基江蓠和及其繁枝变种为同一个种,从而在DNA水平上支持了传统形态分类的观点.
- 细基江蓠 /
- 细基江蓠繁枝变种 /
- RAPD /
- ITS /
- 分类 /
- 鉴定
Abstract: RAPD detection and IT S1,5.8 s rDNA sequencing analysis were conducted to study the genetic variation and phylogenesis of Gracilaria tenuistipitata and G.tenuistipitata var.liui.The proportion of polymorphic,average heterozygosity and genetic distance were compared,a cluster tree was constructed by UPGMA from TFPGA. The results show that the genetic diversity of G.tenuistipitata is higher than G.tenuistipiitata var.liui.Eight RAPD primers were selected through the screening from the 300 primers,and its yielded specific markers for verification of G.tenuistipitata and G.tenuistipitatavar.liui.Only one base difference was found from the IT S1 and 5.8srDNA sequences analysis between G.tenuistipitata and G.tenuistipitata var.liui.it is indicated that RAPD method can be used for Gracilaria germplasm verification and genetic analysis,and it is confirmed that G.tenuistipitata and G.tenuistipitata var.liui belong to the same species,which meant that G.tenuistipitata var. liui is the morphological variant of G.tenuistipitata.
- Gracilaria tenuistipitata /
- G.tenuistipitata var.liui /
- RAPD detection /
- ITS sequences analysis /

HTML全文

参考文献(26)

夏邦美.中国藻类志[M].北京:科学出版社,1999.17-77.

陈昌生,章景荣.福建省细基江蓠繁枝变型人工栽培的探讨[J].集美大学学报(自然科学版),1999,4(2):65-71.

刘思俭.我国江蓠的种类和人工栽培[J].湛江海洋大学学报,2001,21(3):71-79.

周歧存,叶富良,刘东超,等.细基江蓠繁枝变种与细基江蓠营养成分的比较研究[J].海洋科学,2001,25(4):11-13.

张竣甫,夏邦美.中国江蓠属海藻的分类研究[J].海洋科学集刊,1976,11:91-163.

吴超元,李纫芷,林光恒,等.细基江蓠繁枝变型生长适宜环境条件的研究[J].海洋与湖沼,1994,25(1):60-66.

赵素芬,庄承纪.两种江蓠藻体的切断再生[J].湛江海洋大学学报,1999,19(2):25-29.

刘静文,董双林等.温度和盐度对几种大型海藻生长率和NH4-N吸收的影响[J].海洋学报,2001,23(2):109-116.

许忠能,林小涛,林继辉,等.营养盐因子对细基江蓠繁枝变种氮磷吸收速率的影响[J].生态学报,2002,22(3):366-374.

刘思俭,揭振英,曾淑芳.细基江蓠密集型采孢子培育幼苗试验[J].湛江水产学院学报,1990,10(2):89-93.

林光恒,张京浦,许实荣,等.细基江蓠繁枝变种北移越冬研究[J].海洋科学,1993,7(4):3-5.

王焕明,李少芬,陈浩如,等.江蓠与新对虾.青蟹混养试验[J].水产学报,1993,17(4):273-281.

汤坤贤,袁东星,林泗滨,等.江蓠对赤潮消亡及主要水质指标的影响[J].海洋环境科学,2003,22(2):24-27.

赵谋明,邱慧霞,彭志英.江蓠琼胶提胶新工艺的研究[J].食品与发酵工业,1996,23(2):3-7.

贾建航,王萍,金德敏,等.RAPD标记在紫菜遗传多样性检测和种质鉴定中的应用[J].植物学报,2000,42(4):403-407.

岳志芹,李大鹏,戴继勋.应用RAPD技术对裙带菜的遗传分析[J].海洋通报,2000,19(6):24-28.

SOSA S. Genetic variability of Gracilaria cervicornis (Rhodophyta) gametophytes from the Carnary Islands[J]. Eur J Phycol, 1996, 31: 143-147.

SANTELICES B. Strain selection of clonal seaweeds[A]. ROUND FE, CHAPMAN DJ, ed. Progress in Phycological Research: Vol. 8[M]. Bristol: Biopress Ltd, 1992. 85-116.

TSENG C K, XIA B M. On the Gracilaria in the western Pacific and the southeastern Asia region[J]. Botanica Marina,1999, 42: 209-217.

GOFF L J. Molecular delineation of species and species relationships in the red algal agarophytes Gracilariopsis and Gracilaria (Gracilariales)[J]. J Phycol, 1994, 32: 521-537.

GONZALAZ M, ROLANDO M, CANDIA A. Organellar DNA restriction fragment length polymorphism (RFLP) and nuclear random amplified polymorphic DNA(RAPD) analyses of morphotypes of Gracilaria (Gracilariales, Rhodophyta) from Chile[J]. Hydrobiologia, 1996, 326/327: 229-234.

BELLORIN A M. Phylogeny and systematics of the marine algal family Gracilariaceae (Gracilariales, Rhodophyta) based on small subunit rDNA and ITS sequences of Atlantic and Pacific species[J]. J Phycol, 2002, 38: 551-563.

GURGEL C F D. Systematic of Gracilariopsis (Gracilariales, Rhodophyta) based on rbcL sequence analysis and morphological evidence[J]. J Phycol, 2003, 39: 154-171.

MENENSES I. Assessment of populations of Gracilaria chilensis (Gracilariales, Rhodophyta) utilizing RAPDs[J]. J Appl Phycol, 1996, 8: 185-192.

LIMP E, THONG K L, PHANG S M. Molecular differentiation of two morphological variants of Gracilaria salicornia[J]. J Appl Phycol, 2001, 13: 335-342.

张学成,费修绠.细基江蓠原变型及繁枝变型的培养及杂交实验[B].中国科学院海洋研究所开放室研究年报[R].青岛:青岛海洋大学出版社,1991.67-71.

施引文献

资源附件(0)

访问统计

点击查看大图

计量

文章访问数: 1038
HTML全文浏览量: 20
PDF下载量: 1036
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

留言板