两种新贝尼登虫28S rRNA序列分析及其亲缘关系探讨

张纹; 王军; 苏永全; 丁少雄; 杨文川

两种新贝尼登虫28S rRNA序列分析及其亲缘关系探讨

1.
厦门大学, 海洋学系, 福建, 厦门, 361005;厦门大学, 亚热带海洋研究所, 福建, 厦门, 361005
2.
厦门大学, 生物学系, 福建, 厦门, 361005

基金项目: 国家高技术研究发展计划资助项目(863-819-02-12).

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出版历程
- 收稿日期: 2003-02-13
- 修回日期: 2003-09-22

The genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences

1.
Department of Oceanography, Xiamen University, Xiamen 361005, China;Institute of Subtropical Oceanography, Xiamen University, Xiamen 361005, China
2.
Deptartment of Biology, Xiamen University, Xiamen 361005, China

摘要

摘要: 采用一对特定引物对形态相近的单殖吸虫纪新贝尼登虫和梅氏新贝尼登虫的基因组DNA进行PCR扩增,获得其28SrRNA的350bp左右的特异片段.测序后经软件分析发现两者同源的337bp序列仅存在一个碱基的差异,遗传相似度达99.70%,甚至高于不同海区不同宿主梅氏新贝尼登虫间99.41%的相似度,与贝尼登虫属3个虫种的同源序列间的遗传差异度为2.08%～11.73%.通过UPGMA和MP聚类分析也可看出,纪新贝尼登虫和梅氏新贝尼登虫的遗传相似性是极为相近的.虽然两种新贝尼登虫的形态特征和遗传多样性极为相似,但它们是否同属于一个种还不能确定,需要经进一步的形态系统比较和更多的基因分析才能得出客观的、公认的结果.
- 28SrRNA /
- 贝尼登虫 /
- 序列分析
Abstract: The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda,Neobenedenia girellae and N.melleni were obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced.The results show that the comparison of 28S rRNA sequences,with only a base varies in 337 bp accounting for 0.3% genetic difference,the relative species N.girellae and N.melleni parasitized on the different fishes in different farms display that they possess very high genetic similarity of 99.7%,higher than that of 99.41% for the single species N.melleni cultured in different area,and the intraspecific divergence of N.melleni is 0.59%.Meanwhile,the interspecific differences between the two Neobenedenia and three Benedenia (i.e.B.lutjani,B.rohdei and B.seriolae) range from 2.08% to 11.73%.In addition,UPGMA and MP molecular phylogenetic trees were constructed and proved to be consistent with each other.Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show the high similarity,whether they belong to a single species or not is still undefined,the more genes of them should be further investigated,combining with the systematicall and detailed morphological study.
- 28S rRNA /
- Neobenedenia /
- sequence analysis

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参考文献(13)

YAMAGUTI S.Systema Helminthum:Vol.Ⅳ.Monogenea and Aspidocotylea [M].New York:John Wiley and Dons Interscience Publishers,1963.699.

WHITTINGTON I D,HORTON M A.A revision of Neobenedenia Yamaguti,1963 (Monogenea Capsalidae) including a redescription of N-melleni (MacCallum,1927) Yamaguti[J].Journal of Natural History,1996,30(8):1 113-1 156.

BONDADREANTASO M G,OGAWA K,FUKUDOME M.Reproduction and growth of Neobenedenia girellae (Monogenea,Capsalidae):a skin parasite of cultured marine fishes of Japan[J].Fish Pathology,1995,30(3):227-231.

KOESHARYANI I,ZAFRAN,YUASA K,et al.Two species of Capsalid monogeneans infecting cultured humpback grouper Cromileptes altivelis in Indonesia[J].Fish Pathology,1999,34(3):165-166.

YOSHINAGA T,NAGAKURA T,OGAWA K,et al.Attachment-inducing capacities of fish tissue extracts on oncomiracidia of Neobenedenia girellae (Monogenea,Capsalidae) [J].Journal of Parasitology,2000,86(2):214-219.

萨姆布鲁克J,弗里奇E F,曼尼阿蒂斯T.分子克隆实验指南(第二版)[M].北京:科学出版社,1995.

HASSOUNA N,MICHOT B,BACHELLERIE J-P.The complete nucleotide sequence of mouse 28S rRNA gene:implications for the process of size increase of the large subunit rRNA in higher eukaryotes [J].Nucleic Acids Res,1984,12:3 563-3 583.

ISABELLE Mollaret,BARRIE G M,JAMIESON Jean-Lou Justine.Phylogeny of the Monopisthocotylea and Polyopisthocotylea (Platyhelminthes) inferred from 28S rDNA sequences[J].International Journal for Parasitology,2001,30:171-185.

PHILIPS R B,PLEYTE K A,BROWN M R.Salmonid phylogeny inferred from ribosomal DNA restriction maps[J].C J Fish Aquat Sxi,1992,49:2 345-2 353.

GERBIS.The evolution of eukaryotic ribosomal DNA[J].Biosystems,1986,19:247-258.

HASEGAWA M,IIDA Y,YANO T,et al.Phylogenetic relationshipis among eucaryotic kingdoms inferred from ribosomal RNA sequences[J].J Mol Evol,1985,22:32-38.

屈良鹄,陈月琴.物分子分类检索表——原理与方法[J].中山大学学报(自然科学版),1999,38(1):1-6.

张纹,王军,苏永全,等.海水养殖鱼类新本尼登虫的RAPD分析[J].海洋学报,2002,24(4):136-140.

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