牙鲆养殖群体遗传结构的研究

黎中宝

牙鲆养殖群体遗传结构的研究

黎中宝

基金项目: 福建省重点科技攻关资助项目(2002N020)

计量
- 文章访问数: 991
- HTML全文浏览量: 11
- PDF下载量: 975
- 被引次数: 0
出版历程
- 收稿日期: 2003-05-20
- 修回日期: 2003-08-25

Genetic structure of cultivated stocks in Paralichthys olivaceus

LI Zhong-bao

摘要

摘要: 应用聚丙烯酰胺凝胶电泳技术研究了牙鲆养殖群体(F1代)的遗传结构.结果表明,牙鲆养殖群体具有较高的遗传多样性水平.牙鲆群体平均每位点等位基因数的变化为1.19(0.11)～1.24(0.10).多态位点百分数的变化为14.29%～23.81%.期望杂合度的变化为0.056(0.036)～0.057(0.033).观察杂合度的变化为0.099(0.065)～0.104(0.065).牙鲆群体间遗传分化较低,群体间分化度为0.005,表明总的遗传变异中有99.5%来自群体内,有0.5%来自群体间.群体间遗传距离和遗传一致度均值分别为0.001和0.999.群体间基因流很大,等于49.75.
- 牙鲆 /
- 等位酶 /
- 遗传结构 /
- 遗传多样性 /
- 遗传分化
Abstract: Genetic structure of cultivated stocks was investigated using the assay of vertical slab polyacr ylamidegel electrophoresis in Paralichthys olivaceus.Higher level of genetic diversity in the stocks was found:Mean number of alleles perlocus(A) is 1.19(0.11)~1.24(0.10),percentage of polymorphic loci(P_0.99) is 14.29%~23.81%, the observed heterozygosity(H_o) is 0.099(0.065)~0.104(0.065),expected heterozygosity(H_e) is 0.056 (0.036)~0.057(0.033).The results showed that the coefficient of gene differentiation between the stocks is low,F_st=0.005,there is no significant difference in biochemical genetic character istics among the stocks.The genetic distance and genetic identity is 0.001 and 0.999 respectively.Gene flow among the stocks is large(Nm=49.75).
- Paralichthys olivaceus /
- allozyme /
- genetic structure /
- genetic diversity /
- genetic differentiation

HTML全文

参考文献(25)

尤锋,王可玲,相建海,等.山东近海牙鲆同工酶的生化遗传分析[J].海洋与湖沼,1999,30(2):127-133.

王可玲,尤锋,徐成,等.5种海水鱼同工酶组织表达的特性及其电泳的初步分析[J].海洋与湖沼,1996,27(6):626-631.

KIM D S,CHEONG S C,PARK S R,et al. Cytogenetic and biochemical studies on the flatfish,Paralichthys olivaceus[J].Bull Natl Fish Dev Agency (Korea),1988,42:135-142.

黎中宝.牙鲆群体生化遗传学研究.Ⅰ.组织特异性研究[J].中国生态农业学报,2003,11(2):1-4.

黎中宝,邹志华,常建波.牙鲆群体生化遗传学研究.Ⅱ.等位酶的遗传分析[J].中国生态农业学报,2003,11(3):9-12.

莽克强,徐乃正,方荣祥.聚丙烯酰胺凝胶电泳[M].北京:科学出版社,1975.26-47.

朱蓝菲.鱼类同工酶和蛋白质的聚丙稀酰胺梯度凝胶电泳法[J].水生生物学报,1992,16(2):183-185.

HARRIS H,HOPKINSON D A. Handbook of Enzyme Electrophoresis in Human Genetics[M].Amsterdam,Oxford:North Holland Pulishing Com,1976. 66-162.

曾呈奎,相建海.海洋生物技术[M].济南:山东科技出版社,1998.269-282.

王中仁植物等位酶分析[M].北京:科学出版社,1996 77-119.

SHAKLEE J B,ALLENDORF F W,MORIZOT D C,et al. Genetic nomenclature for protein-coding loci in fish [J].Trans Amer Fish Soci,1990,119:2-15.

SWOFFORD D L,SELANDER P K. A computer program for the analysis of allelic variation in population genetics and biochemical systematics[J].J Hered,1989,72:281-283.

NEI M. Genetic distance between populations[J].Amer Natu,1972,106:283-292.

黎中宝,吴仲庆.几种优良养殖对虾杂合性的比较研究[J].海洋科学,2002,26(12):45-48.

徐成,王可玲,张培军.鲈鱼群体生化遗传学研究.Ⅱ种群生化遗传结构及变异[J].海洋与湖沼,2001,32(3):248-254.

SELANDER R K. Genetic variation in natural populations[A].AYALA F J,ed. Molecular Evolution[M].Massachusetts:Sinauer Associates,1976. 21-45.

王可玲,张培军,刘兰英,等.中国近海带鱼种群生化遗传结构及其鉴别的研究[J].海洋学报,1994,16(1):93-104.

王继伟,孔杰,庄志猛,等.真鲷野生群体和人工繁殖群体的同工酶遗传差异[J].生物多样性,2000,8(4):391-396.

GELLENSTEN U. The genetic structure of fish:differences in the intarspecific distribution of biochemical genetic variation between marine,anadromous,and freshwater species[J].J Fish Biol,1985,26(6):691-699.

BUTH D G. Allozymes of the cyprinid fishes:variation and application[A].TRUNER B J,ed. Evolutionary Genetic of Fisfes[M].New York,London:Plenum Press,1983. 561-590.

KIRPICHNIKOV V S. Genetic Bases of Fish Selection[M].Berlin,Heidelbarg,New York:Springer-Verlag,1981.143-200.

李思发,吴力钊,王强,等.长江、珠江、黑龙江鲢、鳙、草鱼种质资源研究[M].上诲:上海科学技术出版社,1990. 51-101.

SHAKLEE J B,SALINI J P. Genetic variation and population subdivision in Australian Barramundi,Lates calcarifer(Bloch) [J].Aust J Mar Fish Res,1985,36:203-218.

李思发.鱼类繁育群体遗传性能的保护[J].水产学报,1988,12(3):283-290.

NEI M. Molecular evolutionary genetics[M].New York:Columbia University Press,1987. 512.

施引文献

资源附件(0)

访问统计