节旋藻和螺旋藻对7种抗生素敏感性的比较研究

臧晓南; 张学成; 王高歌; 刘金姐; 张晓辉; 刘滨

节旋藻和螺旋藻对7种抗生素敏感性的比较研究

基金项目: 国家海洋"863"计划资助项目(819-04-10)

计量
- 文章访问数: 1032
- HTML全文浏览量: 16
- PDF下载量: 1618
- 被引次数: 0
出版历程
- 收稿日期: 2002-07-29
- 修回日期: 2003-07-31

Comparative study on sensitivities of Arthrospira platensis and Spirulina subsulsa to seven antibiotics

摘要

摘要: 对两种丝状蓝藻(钝顶节旋藻和盐泽螺旋藻)在基因工程中常用作选择试剂的7种抗生素——氯霉素、氨苄青霉素、红霉素、链霉素、卡那霉素、庆大霉素和新霉素的敏感性作比较实验.结果表明,两种蓝藻对抗生素的敏感性既有共同的特点,也有明显的差异.它们对红霉素、氯霉素和链霉素最敏感,致死浓度分别为0.1,0.5和5μg/cm³.两种蓝藻对氨苄青霉素比较敏感,1μg/cm³的氨苄青霉素即可抑制Arthrospira.341和Spirulina.351的生长,但6d后生长恢复.Arthrospira.341和Spirulina.351对卡那霉素、庆大霉素和新霉素均有抗性,而且存在很大差异:300μg/cm³的卡那霉素对Arthrospira.341的生长仍然没有影响,但对于Spirulina.351,50μg/cm³的卡那霉素即对其生长有明显抑制作用;200μg/cm³的卡那霉素即可将其全部致死.200μg/cm³的庆大霉素和300μg/cm³的新霉素不能抑制Arthrospira.341和Spirulina.351的生长,但在这两种抗生素环境中两种藻的生长状态有很大差异.并验证了氯霉素、红霉素和链霉素是节旋藻和螺旋藻基因转化过程中的有效的抗性选择剂,也从对抗生素敏感性方面表明节旋藻和螺旋藻两个属的遗传差异.
- 节旋藻 /
- 螺旋藻 /
- 选择标记 /
- 抗生素 /
- 敏感性
Abstract: The sensitivities of Arthrospira platensis FACHB341 and Spirulina subsulsa FACHB351 to seven antibiotics-chloramphenicol(Cm),erthromycin(Em),ampicillin(Amp),streptomycin(Str),kanamycin(Km),gentamycin(Gm) and neomycin(Nm) are presented.The results reval that sensitivities of the two species to the seven antibiotics show a similar tendency,however significant difference still exists.It is provedthat the two specis of blue-green algae are all highly sensitive to Em,Cm and Str.0.1 μg/cm³,0.5 μg/cm³ Cm or 5 μg/cm³ is the lethal concentration to them.A.341 and S.351 can be inhibited by 1 μg/cm³ Amp,and then they recovered after 6 d.A. 341 and S.351 are not sensitive to Km,Gm and Nm,Besides the difference of sensitivities between the two algae is great.A.351 cannote inhibited by even 300 μg/cm³ Km,whereas to S.351,50 μg/cm³ Km can obviously in-hibit the growth of it and 200 μg/cm³ Km can kill it.200 μg/ml Gm and 300 μg/cm³ Nm cannotinhibit the growth of these two species.It is suggested that Cm,Em and Strare useful selective markers for genetransfer of A.341 and S.351.It is also shown that the significant difference between A.341 and S.351 maybe is based on their genetic discrepancy.
- Arthrospira platensis /
- Spirulina subsulsa /
- selective marker /
- antibiotics /
- sensitivity

HTML全文

参考文献(23)

曹淑莉,向葆卿.8种海洋饵料微藻蛋白质含量及氨基酸组成比例的比较研究[J].海洋学报,1993,15(4):98-103.

陈峰,姜悦.微藻生物技术[M].北京:中国轻工业出版社,1999.

VONSHAK A. Spirulina platensis(Arthrospira):physiology,cell biology and biotechnology[M].Taylor&Francis Ltd,1997.

NELISSEN B,WILMOTTE A,NEEFS J-M,et al. Phylogenetic relationship among filamentous helical cyanobacteria investigated on the basis of 16S ribosomal RNA gene sequence analysis system[J].Appl Microbiol,1994,17:206-210.

茅云翔,杨官品,张宝红,等.16SrRNA基因与16S-23SrRNA转录单元内间隔区序列分析及其在节旋藻和螺旋藻分类鉴定中的应用[J].高技术通讯,2001,6:12-18.

王关林,方宏筠.植物基因工程原理与技术[M].北京:科学出版社,1998.167-174.

THIEL T,POOH. Transformation of a filamentous cyanobacteria by electroporation[J].J Bacterial,1989,171:5 743-5746.

CHAUVAT F,ASTIER C,VEDEL F,et al. Transfornation in the cyanobacterium Synechococcus R2:improvement of efficiency;role of the pUH24 plasmid[J].Mol Gen Genet,1983,191:39-45.

KAREN S Kolowsky,JOHN G K Williams,ALADAR A Szalay. Length of foreign DNA in chimeric plasmids determines the efficiency of its integration into the chromosome of the cyanobacterium Syrnechococcus R2 [J].Gene,1984,27:289-299.

BUZBY J S,PROTER R D,STEVENS S E Jr. Expression of the Escherichia coli LacZ gene on a plasmid vector in a cyanobacterium[J].Science,1985,230:805-807.

MARSAC N T,TORRE F,SZULMAJSTER J. Expression of the larvicidal gene of Bacillus sphaericus 1593M in the cyanobacterium Anacystis nidulans R2[J].Mol Gen Genet,1987,209:396-398.

STEVENS S E,PORTER R D. Transformation in Agmemellum quadruplicatum [J].Proc Natl Acad Sci USA,1980,77:6 052-6 056.

TAKEYAMA H,TAKEKEMURA N,MATSUNAGE T. Transformation of marine cyanobacteria Synechococcus sp. by electroporation. Current Topics in Marine Biotechnology[M].Fuji Technology Press Ltd,198. 159-160.

CAO Ji-xiang,XU Zeng-fu,QIU Guo-hua,et al. Studies on the sensitivity of Spirulina platensis to antibiotics and herbicide:relationship with selectable markers for genetic transformation[J].Bioresource Technology,1999,70:89-93.

王高歌,臧晓南,张宝红,等.螺旋藻对6种抗生素的敏感性研究[J].高技术通讯,2001,7:6-13.

ZARROUK C. Contribution ãl'étude d'une cyanophycée influencéde divers facteurs physiques la croissance et la photosynthése de Spirulina maxima (Setch et Gardner) Geitler[D].Paris:University of Paris,1966.

萨姆布鲁克(J),弗里奇E F,曼尼阿蒂斯T.分子克隆实验指南(第二版)[M].北京:科学出版社,1996.912.

LU F,VONSHAIC A,BOUSSIBA S,et al. Effect of temperature and irradiance on growth of Haematococcus pluvia(Chlorophyceae) [J].J Phycol,1994,30:829-833.

NAP J P,BIJVOET J,STIEKEMA W J. Biosafety of kanamycin-resistant transgenic plants[J].Transgenic Res,1992,11:239-249.

METZ J G,NIXON P J,ROGNER M,et al. Directed alteration of the D1 polypeptide of photosystem. Ⅱ. evidence that tyrosine-161 is the redox component,Z. connecting the oxygen-evolving complex to the primary electron donor P680[J].Biochemistry,1989,28:6 960-6 969.

MURPHY R C,GASPARICH G E,BRYANT D A,et al. Nucleotide sequence and further characterization of the Synechococcus sp. Strain PCC7002 recA gene:complementation of a cyanobacterial recA mutation by Escherichia coli recA gene[J].J Bact,1990,172:967-976.

Van der PLAS J,HEGEMAN H,de VRIEZE G,et al. Genomic intergration system based on pBR322 sequences for the cyanobacterium Synechococcus sp. PCC7942:transfer of genes encoding plastocyanin and ferredoxin[J].Gene,1990,95:39-48.

WU X,VENNISON S J,LIU H,et al. Mosquito larvicidal activity of transgenic Anabaena strain PCC7120 expressing combinations of genes from bacillus thuringiensis subspisraelensis[J].Appl Envir Microbiol,1997,63:4 971-4 975.

施引文献

资源附件(0)

访问统计