我国沿海三个文蛤地理群的RAPD分析

沈怀舜; 朱建一; 丁亚平; 陈国耀; 陆勤勤; 朱庙先; 许璞

我国沿海三个文蛤地理群的RAPD分析

江苏省海洋水产研究所, 江苏, 南通, 226007

基金项目: 江苏省科技厅1999科技储备资助项目(9900010)

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出版历程
- 收稿日期: 2001-11-30
- 修回日期: 2002-12-05

RAPD analysis of three wild stocks of Meretrix meretrix in China’s coastal seas

Marine Fisheries Institute of Jiangsu Province, Nantong 226007, China

摘要

摘要: 利用RAPD技术分析了我国沿海辽宁、江苏、广西省3个文蛤地理群的遗传变异,总共用60个随机引物进行了RAPD扩增,其中50个随机引物得到了扩增产物,选取其中15个随机引物的扩增图谱进行相似性系数和遗传距离的计算,结果为辽宁(LW)、江苏(JW)、广西(GW)群体内的遗传距离分别为:0.169±0.034,0.117±0.032,0.108±0.031.LW,JW之间为0.222±0.036;JW,GW之间为0.209±0.043;LW,GW之间为0.316±0.047,同时发现引物s396-900bp的标记为JW和LW所特有;引物s433-250bp标记为JW和GW所特有,由此可作为这3个地理群的分子标记.
- RAPD /
- 文蛤 /
- 分子标记 /
- 遗传距离
Abstract: Genetic variation of three wild stocks of Meretrix meretrix was analysed using random amplified polymorphic DNA(RAPD)markers,50 primers got amplified bands in total of 60 primers,amplified bands of 15 primers were selected as statistic similarity index and genetic distances.Genetic distances of intra stock of Liaonin,Jiangsu Province and the Zhuang Autonomous Region of Guangxi are 0.169±0.034,0.117±0.032 and 0.108±0.031,respectively;and those of inter stock of Liaonin-Jiangsu,Jiangsu-Guangxi and Guangxi-Liaonin are 0.222± 0.036,0.209±0.013 and 0.316±0.047,respectively,the results show that genetic distances of three stock in crease with distance increase.The marker of s396-900 bp is owned by Jiangsu and Liaonin stocks,and marker of s433-250 bp is owned by Jiangsu and Guangxi stocks,three stocks were indentified by this two markers.
- RAPD /
- Meretrix meretrix /
- molecular marker /
- genetic distance

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参考文献(14)

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