我国沿海三个文蛤地理群的RAPD分析

沈怀舜; 朱建一; 丁亚平; 陈国耀; 陆勤勤; 朱庙先; 许璞

我国沿海三个文蛤地理群的RAPD分析

江苏省海洋水产研究所, 江苏, 南通, 226007

基金项目: 江苏省科技厅1999科技储备资助项目(9900010)

计量
- 文章访问数: 862
- HTML全文浏览量: 7
- PDF下载量: 1336
- 被引次数: 0
出版历程
- 收稿日期: 2001-11-30
- 修回日期: 2002-12-05

RAPD analysis of three wild stocks of Meretrix meretrix in China’s coastal seas

Marine Fisheries Institute of Jiangsu Province, Nantong 226007, China

摘要

摘要: 利用RAPD技术分析了我国沿海辽宁、江苏、广西省3个文蛤地理群的遗传变异,总共用60个随机引物进行了RAPD扩增,其中50个随机引物得到了扩增产物,选取其中15个随机引物的扩增图谱进行相似性系数和遗传距离的计算,结果为辽宁(LW)、江苏(JW)、广西(GW)群体内的遗传距离分别为:0.169±0.034,0.117±0.032,0.108±0.031.LW,JW之间为0.222±0.036;JW,GW之间为0.209±0.043;LW,GW之间为0.316±0.047,同时发现引物s396-900bp的标记为JW和LW所特有;引物s433-250bp标记为JW和GW所特有,由此可作为这3个地理群的分子标记.
- RAPD /
- 文蛤 /
- 分子标记 /
- 遗传距离
Abstract: Genetic variation of three wild stocks of Meretrix meretrix was analysed using random amplified polymorphic DNA(RAPD)markers,50 primers got amplified bands in total of 60 primers,amplified bands of 15 primers were selected as statistic similarity index and genetic distances.Genetic distances of intra stock of Liaonin,Jiangsu Province and the Zhuang Autonomous Region of Guangxi are 0.169±0.034,0.117±0.032 and 0.108±0.031,respectively;and those of inter stock of Liaonin-Jiangsu,Jiangsu-Guangxi and Guangxi-Liaonin are 0.222± 0.036,0.209±0.013 and 0.316±0.047,respectively,the results show that genetic distances of three stock in crease with distance increase.The marker of s396-900 bp is owned by Jiangsu and Liaonin stocks,and marker of s433-250 bp is owned by Jiangsu and Guangxi stocks,three stocks were indentified by this two markers.
- RAPD /
- Meretrix meretrix /
- molecular marker /
- genetic distance

HTML全文

参考文献(14)

WILLIAMS J G K, KENNETH J A R, et al. DNA polymorphisms anplified by arbitrary printet are useful as genetic markers[J]. Nucleic Acids Res, 1990, 18(22): 6 531-6 535.

宋林生,相建海,李晨曦,等.用RAPD标记研究对虾属六个种间的亲缘关系[J].动物学报,1998,44(3):353-359.

何舜平,汪亚平,陈宜瑜.五种鲤科鱼类的RAPD分析兼论稀有鲫的系统位置[J].水生生物学报,1997,21(13):262-267.

陈洪,杨靖,薛国雄,等.RAPD技术在异精激发方正银鲫比较研究中应用[J].科学通报,1994,39(7):621-623.

贾建航,王萍,金德敏,等.RAPD标记在紫菜遗传多样性检测和种质鉴定中的应用[J].植物学报,2000,42(4):403-407.

王新望,赖菁茹,姚大年,等.中国春Ph1基因的RAPD标记鉴定[J].农业生物技术学报,1999,(1):1-8.

张四明,邓怀,晏勇,等.中华鲟随机扩增多态性DNA遗传多样性研究[J].海洋与湖沼,2000,31(1):1-7.

丁少雄,王军,王成干,等.鮸状黄姑鱼养殖群体的遗传多样性[J].科学通报,1998,43(21):2 294-2 299.

陆朝福,朱立煌.植物育种中的分子标记的辅助选择[J].生物工程进展,1995,15(4):245-252.

NEI M, LI W H. Mathematical model for studing genetic variation in terms of restriction endonuclease[J]. Proc Natl Acad Sci USA, 1979, 76(10): 5 269-5 273.

刘必谦,戴继勋,喻子牛.RAPD标记在大连湾牡蛎种群研究中的应用[J].青岛海洋大学学报,1998,28(1):82-87.

REEB C A, AVISE J C. A genetic discontinuity in a continously distributed species: mitochondria DNA in the American oyster, Crassoatrea virginica[J]. Genetics, 1991, 124: 397-406.

O' FOIGHIL D, GAFFNEY P M, HILBISH T J. Differences in mitochondria 16S ribosomal gene sequences allow discrimination among American (Crassostrea virginca Gmelin) and Asian(C. gigas Thunberg, C. ariakensis Wakiya)oyster specieds[J]. J Exp Biol Ecol, 1995, 192: 200-211.

GARCIA K G. Genetic diversity of cultured Penaeus vannamei shrimp using three molecular genetic techmiques[J]. Molecular Marine Biology and Biotechnology, 1994, 3(5): 270-280.

施引文献

资源附件(0)

访问统计