除草剂San9785在高EPA后棘藻品系筛选中的应用

邹立红; 张学成

除草剂San9785在高EPA后棘藻品系筛选中的应用

邹立红¹,
张学成²

1.
中国科学院, 海洋研究所, 山东, 青岛, 266071
2.
中国海洋大学, 海洋生命学院, 山东, 青岛, 266003

基金项目: 国家海洋“863”高科技计划资助项目(819-02-01)

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出版历程
- 收稿日期: 2002-02-07
- 修回日期: 2002-07-08

Selection of high EPA content strains of Ellipsoidion sp.by using Herbicide San9785

ZOU Li-hong¹,
ZHANG Xue-cheng²

1.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2.
Ocean University of China, Qingdao 266003, China

摘要

摘要: 后棘藻(Ellipsoidion sp.)是一种高脂海洋微藻,采用微生物筛选平板的方法,利用多不饱和脂肪酸EPA合成的阻断剂——除草剂San9785对紫外线诱变的后棘藻细胞进行了高EPA个体的筛选,气相分析结果显示,在检测的8株筛选品系中,5株EPA含量高于出发藻种,平均提高了121.6%,另3株EPA含量低于出发藻种,平均降低的量为12.6%,EPA含量最高的品系达到总脂肪酸的26.22%,比出发藻种提高了212%.提示除草剂San9785可以作为一种高效筛选压力,选育多不饱和脂肪酸含量丰富的微藻品系.
- 除草剂San9785 /
- 品系 /
- 筛选 /
- EPA /
- 后棘藻
Abstract: In order to get high EPA content strains from microalgae Ellipsoidion sp.,the algae were treated by herbicide San9785 which was reported as one of the most effective inhibitors of EPA synthesis,especially in the pathway from C18:2ω-6 to C18:3ω-3.Alga cells were spread on the selective plates which contained San 9785 at the concentration of 0.1 mmol/dm³,after radiated by U.V.light,plates were cultured under optimal conditions.One month later,a few microalgal colonies appeared and were transferred into liquid medium individually.Fourteen strains were selected.The growth rate,total lipids content and fatty acids were compared among these strains vs the initial strain.Results show that EPA content of the selected strains increased as well as the ratio of ω-3 to ω-6 polyunsaturated fatty acids.The highest EPA content in strain E-14 is 26.2% in total fatty acids,212% higher than that of the initial strain,suggesting that herbicide San 9785 is an efficitive and practical chemical compound in selection of high EPA content in microalgae Ellipsoidion sp.
- herbicide San9785 /
- strain /
- selection /
- EPA /
- Ellipsoidion sp.

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参考文献(11)

CHANMUGAM P S,RYAN D H,et al.Fish oil can reduce cardiovascular disease,even with vegetable oil [J].La Agric,1998,41(2):6.

PIETINEN P,ASCHERIO A,KORHON P,et al.Intake of fatty acids and risk of coronary heart disease in a cohort of Finnish men:the alpha-tocopherol,beta-carotene cancer prevention study [J].Am J Epidemiol,1997,145(10):876-887.

ARTS M T,ACKMAN R G,HOLUB B J."Essential fatty acids" in aquatic ecosystems:a crucial link between diet and human health and evolution [J].Can J Fish Aquat Sci/J Can Sci Halieut Aquat,2001,58(1):122-137.

TAYLOR E,COELLO M R,et al.Marketability of fish fed on diets rich in specific dietary lipids and promoted as healthy food [J].European Commission DG 12 Science.Research and Development,1998,5:298-299.

OTERO A,GARCIA D,FABREGAS J.Factors controlling eicosapentaenoic acid production in semi-continuous cultures of marine microalgae [J].J Appl Phycol,1997,9:465-469.

DOHERTY M A,HELLING JS.Biochemical toxicology of herbicide mixtures on Thalassiosira weisflogii [J].Diss Abst Int Pt B-Sci&Eng,1998,58(11):5 869.

SIVAN A,ARAD S.A mutant of the red microalga Porphyridium sp.(Rhodophyceae) resistant to DCMU andatrazine [J].Phycologia,1995,34(4):299-305.

PROVASOLI L.Media and prospects of the cultivation of marine algae.WATENABE A,HAYYORI A.Culture and Collection of Algae [C].Hokone:Jpn Soc Plant Physoil,1968.63-75.

COHEN Z,NOMAN H A,HEIMER Y M.Potential use of substituted pyridazinones for selecting polyunsaturated fatty acid over-producing cell lines of algae [J].Phytochemistry,1993,32(2):259-264.

KHOZIN I,COHEN Z.Differential response of microalgae to the substituted pyridazinone,Sandoz 9785,reveal different pathways in the biosynthesis of eicosapentaenoic acid [J].Phytochemistry,1996,42(4):1 025-1 029.

HENDERSON R J,MACKINLAW E E,HODJSON P.Differential effects of the substituted pyridazinone herbicide Sandoz 9785 on lipid composition and biosynthesis in photosynthetic and non-photosynthetic marine microalgae [J].J Exp Botany,1990,41(227):729-736.

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