高效几丁质降解菌CB101的分离、鉴定及其几丁质酶系的研究
Isolation and identification of a high-efficient chitin-degrading marine bacterium CB101 and studies on its chitinases system
-
摘要: 几丁质是海洋中居首位的多糖物质,它是虾、蟹壳的主要成分,同时广泛存在于昆虫外骨骼及真菌的细胞壁中.许多生物包括植物、细菌、真菌和一些无脊椎动物与脊椎动物都能够产生几丁质酶降解几丁质,其中细菌利用几丁质酶降解几丁质作为生活所需碳源和氮源.从生态角度来看,几丁质酶在自然界几丁质循环过程中扮演了重要角色,作为对环境的适应,多种微生物都带有完整的几丁质酶系.
-
Key words:
- aeromonas caviae /
- affinity chromatography /
- chitinases
-
SAITO A, FUJII T, YONEYAMA T, et al. High-multiplicity of chitinases genes in Streptomyces coelicolor A3 (2)[J].Biosci Biotechnol Biochem, 1999, 63(4):710-718. SUZUKI K, TAIYOJI M, SUGAWARA N, et al. The third chitinase gene(chiC) of Serratia marcescens 2170 and the relationship of its product to other bacterial chitinases[J]. Biochem J, 1999, 343:587-596. LOWRY O H, ROSEBROUGH N J, FARR A L, et al. Protein measurement with the Folin phenol reagent[J]. J Biol Chem,1951,193:265-275. SAMBROOK J, FRITSCH E F,MAIATIS T.分子克隆实验指南[M].金冬雁等译.第二版.北京:科学出版社,1992. WATANABE T, OYANAGI W, SUZUKI K, et al. Chitinase system of Bacillus circulans WL-12 and importance of chitinase A1 in chitin degradation[J]. J Bacteriol, 1990, 172(7):4 017-4 022. ENRICO C. Chitinase from Serratia marcescens[Z]. Methods in Enzymology, Vol. 161[M]. Academic Press, 1988,460-462. SCHREMPF H. Characteristics of chitin-binding proteins from Streptomycetes[J]. Review EXS, 1999, 87:99-108. -
计量
- 文章访问数: 857
- HTML全文浏览量: 5
- PDF下载量: 1665
- 被引次数: 0
下载:
