棕囊藻赤潮原因种的分子鉴定和起源分析

陈月琴; 王宁; 周惠; 屈良鹄; 杨练锋; 吕颂辉; 齐雨藻

棕囊藻赤潮原因种的分子鉴定和起源分析

1.
中山大学基因工程教育部重点实验室, 广东, 广州, 510275
2.
国家海洋局南海环境监测中心, 广东, 广州, 510300
3.
暨南大学水生生物研究所, 广东, 广州, 510632

基金项目: 国家自然科学基金"九五"重大资助项目(39790110);国家自然科学基金资助项目(39970063);广东省自然科学基金资助项目(001213)

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出版历程
- 收稿日期: 2001-10-18
- 修回日期: 2002-01-21

Molecular identification and origin analysis on “red-tide” related Phaeocystis causative species

摘要

摘要: 测定了发生于我国东南海域赤潮原因种Phaeocystis sp.及相关种类P.globosa和P.pouchetii 18S rDNA序列,并以Phylip35分析软件构建序列距离矩阵和分子系统发育树图.结果表明,该赤潮原因种与球形棕囊藻P.globosa 18S rDNA序列完全相同,从分子水平鉴定了该原因种为P.globosa.分子系统发育树图表示的P.globosa和P.pouchetii的分化顺序表明,分布于我国东南海域的P.globosa可能是一种本地起源的暖水种.Phaeocystis globosa不同株系的形态差异,可能是适应不同生活环境的结果.根据分子生物学的数据对有害赤潮藻类进行系统发育分析,并设计用于有害赤潮藻快速鉴定的特异性分子探针,对赤潮的监控和防治具有重要意义.
- 棕囊藻 /
- 赤潮原因种 /
- 18S /
- rDNA
Abstract: The 18S rRN Agenes of "red-tide" related Phaeocystis sp.from the southeast coast of China,and its related species P.globosa and P.pouchetii are sequenced.Phylogenetic trees are constructed by using these 18S rDNA sequences.It is clearly shown that Phaeocystis sp.from the southeast coast of China is remarkably similar to several isolates of P.globosa rather than P.p ouchetii.The results demonstrate that the bloom-forming species of Phaeocystics most likely originated from an endemic warm-water species.The phenotypes of different members of the genus Phaeocystis are variable,apparently changing in response to environmental conditions.
- Phaeocystis /
- “red-tide” causative species /
- 18S Rdna /

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参考文献(10)

黄楚光,李锦蓉,杨炼锋,等.1997年粤东海域水文环境与赤潮关系研究[A].梁松.南海资源与环境研究文集[C].广州:中山大学出版社,1999.

JAHNKE J, BAUMANN. Differentiation between Phaeocystis pouchetii (Hat.) Lagerheim and Phaeocystis globlsaScherffel. I. Colony shapes and temperature tolerances[J]. Hydro Bull, 1987, 21:141-147.

SOURNIA A. Phaeocystis(Prymnesiophyceae):how many species[J]. Hova Hedwigia, 1988, 47:211-217.

陈月琴,屈良鹄,邱小忠,等.甲藻单个细胞DNA的制备及其在赤潮藻分子鉴定中的应用[J].中山大学学报(自然科学版),1997,36(4):66-69.

KIMURA M. A simple method for estimating evolutionary rates of base substitution through comparative studies ofnucleotide sequences[J]. J Mol Evo, 1980, 16:111-120.

KORNMANN P. Beobachtungen an Phaeocystis-kuoturen[J]. Helgolander Wissenchaftlich Meeresuntersuchungen, 1995,5:218-233.

BAUMANN M E M, LANCELOT C, BRANDINI F P, et al. The taxonomic identity of the cosmopolitan prymnesiophytePhaeocystis:a morphological and ecophysiological approach[J]. J Mar Sys, 1994, 5:5-22.

MEDLIN L K, LANGE M, BAUMANN M E M. Genetic differentiation among three colony-forming species ofPhaeocystis:further evidence for the phylogeny of the Prymnesiophyta[J]. Phycologia, 1994, 33(3):199-212.

OCHMAN H, WILSON A C. Evolution in bacteria:evidence for a universal substitution rate in cellular genomes[J]. J MolEvol, 1987, 26:74-86.

FRAKES L A, FRANCIS J E, SYKTUS J I. Climate modes of the Phanerozoic[M]. Cambridge:Cambridge UniversityPress, 1992. 274.

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