温度逆境处理提高拟微球藻(Nannochloropsis oculata)EPA含量的研究
Obvious increase of EPA content of Nannochloropsis oculata achieved in temperature stresses
-
摘要: 二十碳五烯酸(eicosapentaenoic acid,EPA)是一种长链多不饱和脂肪酸,是水产养殖动物幼体发育必需脂肪酸之一.富含EPA鲜活微藻、干燥微藻和冷冻微藻是水产养殖动物幼体重要的饵料[1].同时,EPA还能增强水产养殖动物免疫系统功能,提高成活率和抗病力[1~3].由于EPA含量是饵料藻品质的决定因素之一,品种选育、生态调控等提高EPA含量的措施均能提高微藻饵料价值.低温能提高微藻脂肪酸不饱和度,以维持生物膜流动性,抵抗低温伤害[4].另外,我们推测长链脂肪酸有可能提高微藻适应高温环境的能力.
-
Key words:
- microalgae /
- Nannochloropsis oculata /
- eicosapentaenoic acid /
- temperature stress /
- cell shaped feed
-
麦康生,何艮增强鱼类免疫力的饲料生产技术基础[A]曾呈奎,相建海海洋生物技术[M]济南:山东科学技术出版社,1998 544-551. OTERO A, GARCIA D, FABREGAS J. Factors controlling eicosapentaenoic acid production in semicontinuous cultures of marine microalgae[J]. J Appl Phycol, 1997, 9: 465-469. VAZHAPPILLY R, CHEN F. Eicosapentaenoic acid and docosahexaenoic acid production potential of microalgae and their heterotrophic growth[J]. J Am Oil Chem Soc, 1998, 75(3): 393-397. 魏东,张学成.微藻脂肪酸去饱和酶及其基因表达的生态调控研究新进展[J].海洋科学,2000,21(8):42-46 PROVASOLI L. Media and prospects of the cultivation of marine algae[A]. Watenabe A, Hayyori A. Culture and Collection of Algae[M]. US-Japan Conf, Hokone, Jpn Soc Plant Physiol, 1968. 63-75. LEPAGE G, TOY C. Improved recovery of fatty acid through direct transesterification without prior extraction purification[J]. J Lipid Res, 1984, 25:1 369-1 396. 魏东,张学成,邹立红,等.细胞生长时期对两种海洋微藻总脂含量和脂肪酸组成的影响[J]青岛海洋大学学报,30(3):503-509. COHEN Z, VONSHAK A, RICHMOND A. Effect of environmental conditions on fatty acid composition of the red alga Porphyridium cruentum: correlation to growth rate[J]. J Phycol, 1988, 24: 328-332. ZHU C J, LEE Y K, CHAO T M. Effects of temperature and growth phase on lipid and biochemical composition of Isochrysisgalbana TK1[J]. J Appl Phycol, 1997, 9: 451-457. 魏东,张学成,隋正红,等氮源和N/P对眼点拟微球藻的生长、总脂含量和脂肪酸组成的影响[J]海洋科学,2000,21(7):46-51. CHEN F, JOHNS M R. Effect of C/N ratio and areation on the fatty acid composition of heterotrophic Chlorella sorokiniana[J]. J Appl Phycol, 1991, 3: 203-209. REITAN K I, RAINUZZO J R, OLSEN Y. Effect of nutrient limitation of fatty acid lipid content of marine microalgae[J]. J Phycol, 1994, 310: 972-979. ROESSLER P G. Environmental control of glycerolipid metabolism in microalgae: commercial implications and future research directions[J]. J Phycol, 1990, 26: 393-399. COHEN Z, HEIMER Y M. A6 desaturase inhibition: a novel mode of action of norflurazone[J]. Plant Physiology, 1990,93: 347-349. COHEN Z, DIDI S, HEIMER M Y. Overproduction of γ-linoleic and eicosapentaenoic acids by algae[J]. Plant Physiology, 1992, 98: 569-572.
点击查看大图
计量
- 文章访问数: 980
- HTML全文浏览量: 7
- PDF下载量: 1194
- 被引次数: 0