海湾扇贝养殖群体遗传多样性的研究
The genetic diversity of reared populations of bay scallop Argopecten irradians(Lamarck)
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摘要: 用等位基因酶的聚丙烯酰胺凝胶电泳技术,研究了大连沿岸不同生态环境条件下的大李家湾低排筏、大李家湾高排筏、凌水桥、棋盘磨4个海湾扇贝养殖群体的遗传多样性.在4个群体的7种等位基因酶和闭壳肌蛋白中均检测到了22个基因位点,各群体的多态位点比例分别为22.73%,22.73%,22.73%,18.18%(P≤0.95)和27.27%,27.27%,31.82%,36.36%(P≤0.99);杂合度观测值分别为0.064,0.071,0.075,0.069;Hardy-Weinberg平衡偏离指数分别为-0.068,-0.020,-0.032,-0.050;近交系数分别为0.568,0.565,0.487,0.550;位点等位基因有效数分别为1.102,1.109,1.143,1.122;遗传变异综合评价指数分别为0.0060,0.0071,0.0088,0.0073.海湾扇贝养殖群体遗传多样性总体水平较低,但在这4个群体中,凌水桥群体的遗传多样性水平最高,大李家湾高排筏和棋盘磨群体的遗传多样性水平较高,大李家湾低排筏群体遗传多样性水平最低.Abstract: The genetic diversity of four reared populations(Dalijiadipai DLJD,Dalijiagaopai DLJG,Lingshuiqiao LSQ,Qipanmo QPM) of bay scallop Argopecten irradians(Lamarck) at the coastal shellfish farms in Dalian had been investigated based on polyacrylamide gel electrophoresis for allozyme.22 loci in 7 enzymes and general protein were detected.Based on the 22 common loci of samples in four populations,the proportion of polymorphic loci was 22.73%,22.73%,22.73% and 18.18% respectively under the criterion P≤0.95 and 27.27%,27.27%,31.82% and 36.36% respectively under the criterion P≤0.99; the mean heterozygosity observed was 0.064,0.071,0.075 and 0.069 respectively; the deviation index from Hardy-Weinberg equilibrium was-0.068,-0.020,-0.032 and-0.050 respectively; inbreeding coefficient was 0.568,0.565,0.487 and 0.550 respectively; the mean effective number of allele in a locus was 1.102,1.109,1.143 and 1.122 respectively; the comprehensive index of genetic variation was 0.006 0,0.007 1,0.008 8 and 0.007 3 respectively.The genetic diversity of the bay scallop was lower than the average of bivalve.The genetic diversity in LSQ was the highest,in DLJG and QPM was higher,in DLJD was the lowest among the four populations.
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Key words:
- Argopecten irradians /
- allele /
- genetic diversity
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