海洋生境芽孢杆菌(Bacillus spp.)的培养条件及产生的胞外抗菌蛋白
Cultural conditions of marine Bacillus spp. and effect on produced antagonistic protein
-
摘要: 测试了4株海洋芽孢杆菌的培养条件对其产生抗菌蛋白的影响;提取的粗蛋白的性质以及对6种病原指示菌的抑菌活性,结果表明,4株海洋芽孢杆菌在2216E,EMB,CM培养液;15～35℃;NaCl浓度0%～5%;pH3～9的条件下均能生长,适宜生长温度20～28℃;NaCl浓度1%～3%;pH5～8.菌株分别培养84～108h抑菌活性最强.(NH4)2SO4沉淀抗菌粗蛋白的最适饱和度为50%～70%.粗蛋白对病原菌尤其对病原真菌具有强烈的抑制作用.粗蛋白性质稳定,对高温和蛋白酶不敏感.Abstract: The antagonistic protein production, which inhibits 6 species fungi and bacteria from marine Bacillus spp., and the cultural conditions such as temperature, fermentation peried, NaCl concentration and pH of 4 strians marine bacter ia are studied.The result of test shows that suittable factor for both bacteria growth and antimicrobial substance produce are 20~28, 84~108 h, NaCl 1%~3%, pH5~8.Concentration of ammonium sulfate between 50% and 70% is the most fitting saturation for antagonistic protein precipitability.The proteins are insensitive to heat and protein and have high inhibitory value to pathogenetic fungi.
-
Key words:
- marine Bacillus spp /
- cultural conditions /
- antagonistic protein /
-
刘进元.枯草芽孢杆菌LCⅡB抗菌蛋白的纯化及性质[J].生物工程学报,1992,8(3):266～270. 王金生.细菌素在植物细菌病害生防中的应用[J].生物防治通报,1985,1(2):36～40. KENNETH F.Baker,Evolving concepts of biological control of plant pathogens Ann[J].Rev Phytopathol,1987,25:67～85. 郑福寿,陈绍铭.水生微生物学实验法[M].北京:海洋出版社,1990.27. 宋大新,范长胜,徐德强,等.微生物学实验技术教程[Z],上海:复旦大学出版社,1993.254. 陶慰孙.蛋白质分子基础[M].北京:高等教育出版社,1981.29～34. 王文仲.应用微生物学-现代生物技术[M].北京:中国医药科技出版社,1996.476～479. WAKAYAMA S,LSHIKAWA F,OSIHI K.Mycocerein a novel antifungal peptide antibiotic produced Bacillus cereus Antimicrob[J].Agents Chemother,1984,26(6):939～940. -
计量
- 文章访问数: 744
- HTML全文浏览量: 6
- PDF下载量: 1290
- 被引次数: 0
下载:
