用于紫菜无性系种质鉴定的计算机化DNA指纹的建立

贾建航; 陈一华; 石金锋; 金德敏; 许璞; 梅俊学; 翁曼丽; 王斌

用于紫菜无性系种质鉴定的计算机化DNA指纹的建立

1.
中国科学院遗传研究所, 北京 100101
2.
江苏海洋水产研究所, 江苏南通 226007
3.
中国科学院海洋研究所, 山东青岛 266071

基金项目: 国家“863”计划海洋领域资助项目(819-03-02).

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出版历程
- 收稿日期: 2000-06-02
- 修回日期: 2000-08-14

Construction of computerized DNA fingerprinting for identification of Porphyra lines

1.
Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China
2.
Jiangsu Institute of Oceanology and Hydrobiology, Nantong 226007, China
3.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

摘要

摘要: 研究用RAPD技术对条斑紫菜(Porphyra yezoensis)、坛紫菜(P.Haitanensis)、半叶紫菜(P.Katadai var.Hemiphylla)和少精紫菜(P.Oligospermatangia)的15个无性系丝状体进行了遗传多样分析,用120个Operon引物进行了筛选,从中选用来自OPJ-18和OPN-02扩增出的8条多态性条带构建了这15个紫菜无性系的DNA指纹图谱,在该图谱中每个紫菜无性系均有各自特异的DNA指纹.用1和0分别表示DNA带的出现和缺失,将各个无性系的DNA指纹用计算机语言表示,建立了15个紫菜无性系的计算机化DNA指纹;在此基础上设计了紫菜无性系种质鉴定的计算机软件PhGI.
- 计算机化的DNA指纹 /
- 种质鉴定 /
- RAPD /
- 紫菜
Abstract: RAPD(randomly amplified polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing P.Yezoensis,P.Haitanensis,P.Katadai var.hemiphylla and P.oligospermatangia.Eight stable and repeatable RAPD bands amplified with two primers,OPN-02 and OPJ-18,were selected for the construction of DNA fingerprinting.The RAPD results are scored based on the presence or absence of each of the 8 bands and then converted to wmputer language expressed with two digitals,1 and 0,which represent the presence(numbered as 1) or absence(numbered as 0) of each band,respectively.Based on these results,a model DNA fingerprint and a cqmputerized DNA fingerprint are constructed.In the constructed DNA fingerprint,each Porphyra line has its unique fingerprinting pattern and can be easily distinguished from each other.Later,a software,named as PhGI,is designed based on this DNA finaerorintina for Porphyra line identification.
- DNA fingerprinting /
- germplasm identification /
- PAPD /
- Porphyra

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参考文献(10)

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