中国对虾黄、渤海沿岸地理群的RAPD分析
RAPD analysis of wild stock of penaeid shrimp (Penaeus chinensis) in the China’s coastal waters of Huanghai and Bohai Seas
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摘要: 采用随机扩增多态DNA(random amplified polymorphic DNA,简称RAPD)技术,对中国对虾的黄、渤海地理群的32个个体遗传多样性进行了检测,使用OPI系列的20个10bp的寡核苷酸随机引物,对每个个体的基因组DNA进行扩增,结果为:17个引物获得了谱带清晰且重复性好的产物,扩增片段的分子量在2000～2000bp之间,共检测106个位点,其中多态位点数为39个,占36.8%;平均遗传距离为0.0941+0.0206,有67个标记(占63.2%)在整个群体的32个个体间表观稳定的一致性.Abstract: Genetic diversities of 32 individuals of P.Chinensis in the China's coastal waters of the Huanghai and Bohai Seas are detected by RAPD technique.20 decamer primers of OPI are used for DNA amplification for each individual.The results show that of reproducible fingerprints 17 primers are obtained,and the bands are clear.39 of 106 loci detected are polymorphic,amounting to 36.8%.Mean genetic distance is 0.094±0.0206; 68 markers (63.2% of the total) show stable homogeneity in all of the 32 individuals.
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