江蓠属藻胆蛋白的研究──Ⅱ.龙须菜藻胆蛋白光谱特性和荧光动力学研究
Studies on phycobiliprotein in Gracilaria Ⅱ.Characterization on aborption and emission spera and fluorescent dynamics in intact thallus of Gracilaria lemaneiformis
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摘要: 本文以野生型和突变型龙须菜为材料,测定了完整藻体的室温吸收光谱、77K低温荧光发射光谱和叶绿素诱导荧光动力学曲线.野生型(Wt)和黄色突变体(Ye100)的室温吸收光谱有435、498、540、566、618、640和680nm7个吸收峰;绿色突变体Gr180和Gr184则只有除540nm以外的6个吸收峰.3种突变体的藻红蛋白含量均比野生型少,两种绿色突变体的藻蓝蛋白含量相对有所增加,别藻蓝蛋白含量变化不明显.77K低温荧光发射发谱(激发光分别为436、540和580nm)的结果表明:在发射峰的波长上,4种材料没有明显的区别,但Wt中PE吸收的光能传递效率比较低.叶绿素诱导荧光动力学曲线显示Wt的光系统Ⅱ活性、光系统Ⅱ原初光能转化效率及光合碳代谢活性都高于突变体.Abstract: In the present paper, intact living thalli of Gracilaria lemaneiformis,used to measure room temperature.absorption spectra, low temperature (77 K) fluorescent emission spectra, and chlorophyll-inducing fluorescent dyosmic curve.For wild type material (Wt) and yellow mutant (Ye100),there are 7 peaks in the absorption spectra:435. 498. 540. 56B. 618. 640 and 680 nm, and for green mutants Gr180 and Gr184, there are aV the absorption peaks except 540 nm one. The phymerythrin content in three mutants is lower than that in wild type thallus, the comparative content of phycocyanin in gree.mutants is higher than that in others, but there is no obvious change of allophycocyanin content in all the materials. The results of bw temperature (77 K) fluorescent emission indicate that there is no difference for fluorescent emission peaks of the wild type and mutants (the excitation wavelength are 436, 540 and 580 nm respectively,but the transmission effciency of light energy of phycoerythrin in wild type is lower than that of others. The chlorophyll-inducing fluorescent dynamic curve shows that the activity oI PSⅡ,the trans-mission efficiency in primary light energy and the carbon metabolic activity of PSⅡ in wild type thallus are higher than these in three mutants.
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