海藻工具酶研究Ⅱ.褐藻酸酶的制备、性质及对裙带菜细胞的解离研究
Studies on algal tool enzymes Ⅱ.Preparation and some properties of alginase and separation of cells in Undaria pinnatifida by enzymeHan Baoqin
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摘要: 褐藻酸降解菌埃氏交替单胞菌(Alteromonas espejiana)菌株Al01,通过发酵培养制备褐藻酸酶.该酶作用的最适pH为7.0,最适温度为40℃,最适底物浓度为1%～2%;在离子浓度为0.5mmol/dm3时,Mn2+对酶促反应稍有促进作用,Ca2+、Hg2+则有明显的抑制作用.该酶用于裙带菜单细胞和原生质体解离时,以酶液组成为褐藻酸酶1%、纤维素酶1%,45×10-3的NaC1为渗透剂,酶解温度25℃,pH7.0,酶解3-4h效果最好.Abstract: Alginase was prepared by fermenting culture strain Al01 of alginate-degrading bacteria Alteromonas espejiana.The optimum temperature and pH for the enzyme were 40℃ and pH7,respectively.The optimum substratz concentration was 1%-2%.The enzyme activity was inhibited by Ca2+,Hg2+ and slightly activated by Mn2+ at ion concentration being 0.5 mmol/dm3.The optimum conditions of the separating mono-cells and protoplasts in Undaria pinnatifida were enzyme solution consisting of alginase 1%,cellulase 100, NaCl 45×10-3, pH 7.0 and incubation 3-4h at the 25℃.
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Key words:
- Alteromonas espejiana /
- alginase /
- Undaria pinnatifida /
- protoplast
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