从中国对虾分离纯化的一种杆状病毒及其超微结构的研究

石拓; 孔杰; 包振民; 梁兴明; 刘萍; 王崇明

从中国对虾分离纯化的一种杆状病毒及其超微结构的研究

1.
中国水产科学研究院黄海水产研究所, 青岛266071
2.
青岛海洋大学海洋生命学院, 青岛266003

基金项目: 国家攀登计划B资助课题(编号:PDB6-6-1)

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出版历程
- 收稿日期: 1996-05-13
- 修回日期: 1997-03-06

Studies on Purification and ultrastructure of a baculoviral pathogen in Penaeus chinesis

1.
Yellow Sea Fisheries Research Insititute, Qingdao 266071
2.
College of Marine Life Sciences, Ocean University of Qingdao, Qingdao, 266003

摘要

摘要: 从患病和濒死的中国对虾(Penaeus chinensis)头胸部分离提纯出一种杆状病毒,同时经对病虾的鳃、胃和中肠组织进行超薄切片及电镜观察,亦在上述组织的细胞核内发现大小相同、形态一致的大量杆状病毒粒子.成簇的病毒粒子主要在核内装配,并导致细胞结构的病变.病毒粒子大小约为320～400nm×100～130nm,两端钝圆,中部膨大,有囊膜,分内外两后,核衣壳大小为250～300nm×70～100nm.细胞内未观察到有核型多角体或颗粒体类包涵体存在.根据病毒学分类原则,该病毒应属于杆状病毒属无包涵体杆状病毒亚群,即杆状病毒C亚群.
- 中国对虾 /
- 非包涵体杆状病毒 /
- 分离提纯 /
- 电镜观察
Abstract: A kind of baculovirus was islated from the mid-gut gland tissues of sick of morbid Penaeus chinensis.Electron microscopic examination of ultrathin section of the gills, hepatopancrease and mid-gut gland tissues also revealed the presence of rod-shaped baculovirus particles with same sine in the affected cell nuclei.The virion covered with bilaminal envelope was 320-400 nm×100-130 nm in size,whereas the nucleocapsid ranged in the size of 250-300 nm in length and 70-100 nm in breadth respectively.No occlusion bodies have been found in cells.According to the principle of viral classification, this new found virus can probably belong to the non-occluded subgroup of insect baculoviridae,i.e.,C subgroup baculovirus.
- Penaeus chinesis /
- non-occluded baculovirus /
- isolation and purification /
- electronic observation

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参考文献(17)

Couch J A.Frec and occluded virus,similar to baculovirus,in Hepatopancreas of Pink Shrimp.Nature,1974,(217):229-231

WanK Q,Cai S.L,J.The shrimp farminK industry in China.World Ayuaculture,1997,28(1):23-29

黄捷,宋晓玲,于佳等.杆状病毒性的皮下及造血组织坏死—对虾暴发性流行病的病原和病理学.海洋水产研究.1995,16(1):1-10

蔡生力,黄捷.王崇明等.1993-1994年对虾暴发病的流行病学研究.水产学报,1995,(2):112-118

张建红,陈棣华.肖莲春等.中国对虾非包涵体杆状病毒在体内的感染与发生.中国病毒学,1994.,(4) :362-365

彭宝珍.任家鸣,沈菊英等.急性致死性对虾病的杆状病毒病原研究.病毒学报,1995,11 (2):151-156

Bonami J k et ul.Purification and characterization of the infectious hypodermal and haematopoietic necrosis virus of penaeid shrimps.Journal of General Virology,1990,(71):2 647-2 664

吴友吕.王方国,洪健.长毛对虾杆状病毒病研究.电子显微学报,1994,(5):356

杨复华.病毒学.长沙:湖南科学技术出版社,1992,184-200

Laghtner D V,ftedman K M.A baculovirus-caused disease of the penaeid shrimp,PPnueus morzodorz.J.fnvertbr.Pathol.,1981.(38):299-302

Sano T.Ntshimura T,Oguma K et al.Baculovirus infection of cultured Kuruma shrimp Penueus japonicus in Japan.Fish Pathol.,1981.(15):185-191

Laghtner D V et al.New developments in penaeid virology:application of biotechnology in research and disease diagnosis for shnmp virus of concernm the American.Hawaii:Diseases of cultured penaeid shrimp in Asia and the United States,1992.1 33-253

Owens I.Description of the first haemocytic rod-shaped virus from a penaeid prawns.Dis.Aquat.Org.,1993,16(3):217-221

Kasornchandra J,5upamattaya K.Boonyaratpahn S.Partial characterization of yellow head baculovirus (YHV) in black tiger prawn.Phuket Thailand:Seeoud symposium on disease in Asian aquaculture,]993.75

Chang P S,Lo C F,Kou C:H et al.Purification and amplification of DNA from Penaeus monodon-type baculovirus (MBV).Journal of Invertebrate Pathology.1993,(62):116-120

Sano T,Nishimura T.Fukuda H et al.Baculoviral mid-gut necrosis(BMNV)of Kuruma shrimp (Penaeus Japonicus) larvae in Japanese intensive culture systems.Helgolander Meeresunters,1984,(37):255-264

Arunoto M.Yamazaki T.Mizuta Y et al.Characterization and partial cloning of the genomic DNA of a baculovirus from Penaeus Japonicus (PjNOB=BMNV).Aqualculture.1995,(132):213-220

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