摘要:
主要由β-D葡萄糖苷1,3键结合组成的褐藻淀粉,普遍存在于褐藻体内,在褐藻的代谢中占有一定的位置[1],并具有某些药用价值[2],对其含量的测定方法的研究,无论是对褐藻生理的研究,或是开展褐藻的综合利用,均具有实用价值.褐藻淀粉含量的测定方法,主要是基于将其用稀酸水解后,测出葡萄糖含量,而后推算出褐藻中的含量.迄今仍有作者沿用此原理进行测定.用葡萄糖氧化酶,过氧化物酶和联大茴香胺法测定葡萄糖的方法,由于酶的特异性高,只同葡萄糖反应而不受其他还原糖的干扰,精密度及操作步骤也较为理想,早已在医学和工业方面采用,但至今仍未见有试用于褐藻淀粉的含量测定的报导.
Abstract:
Glucose oxidase, peroxidase and dianisidine method may be used to determine laminaran in brown algae. Its procedure was that the seaweed was hybrolyzed and filtrated, then 0.5 ml of filtrate(the concentration of glucose should be from 0.05 mg to 0.25 mg per ml) was added to a test tube and 5ml of an enzyme reagent (22.4 mg glucose oxidase, 0.56 mg peroxidase, 1.2 mg dianisidine citrate, 2 mg citric acid monohydrate and 12 mg sodium citrate dihydrate) was added.The tube was shaken and held for 30 min at 38℃. Then 0.5 ml 1:1 HCl was added.The color was read at 400nm by spectrophotomter. It was found that the relative standard deviation was 2.44%.The seaweed need hydrolysis by 1 N HCl befere it was used. The accuracy of determination was not interfered by the hydrolysis products.
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